Effect of ornithine in parenteral nutrition regimens on difluoromethylornithine-induced platelet suppression and changes in tumor polyamine content

Cancer Investigation
V B Grossie, K Nishioka

Abstract

We have demonstrated that DFMO-induced thrombocytopenia can be ameliorated with concomitant ornithine (Orn) in chow-fed rats; a reversal in DFMO-associated tumor polyamine reduction and antitumor activity, however, was also evident. To determine the effect of Orn in total parenteral nutrition (TPN) regimens on DFMO-induced thrombocytopenia and changes in tumor polyamine concentrations, Ward-colon-tumor-bearing (WCT) rats were given TPN with arginine (ENA) or with ornithine substituted for arginine (ENO) alone or with DFMO (1.5 g/day) added directly to the infusate. After 4 days, the peripheral blood platelet counts for ENA (917 +/- 151 x 10(3)/mm3) or ENO (908 +/- 67 x 10(3)/mm3) were equivalent to those of chow fed rats (901 +/- 42 x 10(3)/mm3). ENA/DFMO rats had significant thrombocytopenia (607 +/- 185 x 10(3)/mm3), which was completely ameliorated for ENO/DFMO rats (939 +/- 111 x 10(3)/mm3). Peripheral white blood count, hematocrit, and other hematological parameters were not affected. Tumor putrescine content for ENA rats (46.9 +/- 8.7 nmol/g) was equal to that for chow-fed rats (44.8 +/- 6.2 nmol/g) and ENO rats (53.6 +/- 8.3 nmol/g). The reduction in tumor putrescine content for ENO/DFMO rats (19.6 +/- 6.9 nmol/g) was eq...Continue Reading

References

Aug 15, 1986·International Journal of Cancer. Journal International Du Cancer·D M OtaK Nishioka
Nov 15, 1988·International Journal of Cancer. Journal International Du Cancer·K NishiokaD M Ota
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T G O'BrienM Holmes
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T G O'BrienM Holmes
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor

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Citations

Oct 31, 2009·Molecular Biology Reports·Wei WangXiu-Li Wang

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