PMID: 6977645Jan 1, 1982Paper

Effect of ouabain, amiloride, and antidiuretic hormone on the sodium-transport pool in isolated epithelia from frog skin (Rana temporaria)

The Journal of Membrane Biology
R Nielsen

Abstract

When tracer Na+ is added to the solution bathing the apical side of isolated epithelia the observed transepithelial tracer influx increases with time until a steady state is reached. The build-up of the tracer flux follows a single exponential course. The halftime for this build-up under control conditions was 0.92 +/- 0.06 min, and in the presence of ouabain 4.51 +/- 0.7 min. It is shown that the calculated Na+-transport pool is located in the cells. The Na+-transport pool under control conditions was 35.6 +/- 3.4 nmol/cm2, which corresponds to an intracellular Na+ concentration of 7.9 mM. Activation of the active Na+ transport by addition of antidiuretic hormone resulted in a highly significant increase in the Na+ transport pool, and inhibition of the transcellular Na+ transport with amiloride resulted in a decrease in the Na+-transport pool. Furthermore, the active Na+ transport increased along an S-shaped curve with increasing intracellular Na+ concentration (Na+-transport pool). The Na+ pump was found to be half saturated at an intracellular Na+ concentration of 12.5 mM.

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Citations

Jan 1, 1986·The Journal of Membrane Biology·J F García-DíazA Essig
Jan 1, 1984·The Journal of Membrane Biology·R S Fisher, K R Spring
Apr 29, 1992·Biochimica Et Biophysica Acta·J EhrenfeldB J Harvey
May 1, 1985·Acta Physiologica Scandinavica·R Nielson
Dec 3, 2011·Zoological Science·Silviya Rajakumari Jared, Jonakuty Prakasa Rao
Jan 1, 1994·Pflügers Archiv : European journal of physiology·B BrodinR Nielsen
Aug 12, 2009·Experimental Physiology·Silviya Rajakumari JaredSathya Subramani
Dec 1, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·E H Larsen, B E Rasmussen
Jul 1, 1987·Pflügers Archiv : European journal of physiology·T C Cox, R E Woods
Jul 7, 1988·Biochimica Et Biophysica Acta·T C Cox
Feb 17, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Erik Hviid LarsenRobert Nielsen
Apr 19, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Scott K ParksGreg G Goss

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