PMID: 6163452Dec 15, 1980Paper

Effect of ouabain on macromolecular synthesis during the cell cycle in mitogen-stimulated human lymphocytes

Biochimica Et Biophysica Acta
M SzamelF Solymosy

Abstract

Ouabain inhibited in a concentration-dependent and completely reversible way, the synthesis of DNA, RNa and protein in phytohemagglutinin and concanavalin A-stimulated human lymphocytes without affecting the uptake of nucleosides and amino acids into the cells. On the other hand, ouabain even at very high concentrations was unable to interfere with the binding of [3H]concanavalin A. No correlation was found between the inhibition by ouabain of macromolecular synthesis and that of K+ transport. The inhibitor effect of ouabain on the stimulation of macromolecular synthesis could be partially reversed by higher concentrations of K+, due to the direct inhibition of ouabain binding. Ouabain added to the cultures at different stages of cell growth suppressed the incorporation of thymidine to various extents. Both ouabain sensitive stages fell in a period preceding the onset of mitosis and were characterized by very active thymidine incorporation. Lymphocytes were most sensitive to ouabain within the S phase. The results suggest that ouabain interferes with mitogen-triggered membrane-associated events, other than K+ transport, controlling mitosis at distinct phases of the cell cycle.

References

Jan 1, 1978·Annual Review of Physiology·J G Kaplan
Jan 1, 1978·Annual Review of Biochemistry·A B PardeeR F Kletzien
Jan 1, 1979·Journal of Cellular Physiology·W Negendank, G Karreman
Dec 1, 1976·The Journal of Clinical Investigation·G B Segel, M A Lichtman
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M Harms-RingdahlL Ehrenberg

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Citations

Jun 1, 1981·Biochemical and Biophysical Research Communications·R S Quinn, G A Rodan
Nov 1, 1995·Journal of Cellular Physiology·C BrodieE W Gelfand
Dec 1, 1996·Japanese Journal of Pharmacology·Y MurataA Baba

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