Effect of ovarian hormones on survival genes in laser captured serotonin neurons from macaques

Journal of Neurochemistry
Cynthia L Bethea, Arubala P Reddy

Abstract

We sought the effect of estradiol (E) and progesterone (P) on survival gene expression in laser captured serotonin neurons and in the dorsal raphe region of monkeys with cDNA array analysis. Spayed rhesus macaques were treated with either placebo, E or E + P via Silastic implant for 1 month prior to killing. First, RNA from a small block of midbrain containing the dorsal raphe was hybridized to Rhesus Gene Chips (n = 3/treatment). There was a significant change in 854 probe sets with E +/- P treatment (anova, p < 0.05); however, only 151 probes sets exhibited a twofold or greater change. Twenty-five genes related to cell survival changed significantly. The expression of vascular endothelial growth factor, superoxide dismutase (SOD1), and the caspase inhibitor, BIRC4, was confirmed with quantitative RT-PCR. Then, RNA from laser captured serotonin neurons (n = 2/treatment) was hybridized to Rhesus Gene Chips. There was a significant change in 744 probe sets, but 10 493 probe sets exhibited a twofold or greater change. Pivotal changes in apoptosis and cell cycle pathways included twofold or greater increases in SOD1, IkappaBalpha, Fas apoptotic inhibitory molecule, fibroblast growth factor-receptor 2 (FGFR2), neurotrophic tyrosine...Continue Reading

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Citations

Jun 7, 2011·Journal of Chemical Neuroanatomy·Matthew W HaleChristopher A Lowry
Apr 28, 2009·Frontiers in Neuroendocrinology·Cynthia L BetheaFernanda B Lima
Dec 22, 2015·Neurobiology of Aging·Cynthia L BetheaHenryk F Urbanski
Mar 14, 2012·Journal of Neuroscience Research·Cynthia L Bethea, Arubala P Reddy
Dec 14, 2011·Molecular and Cellular Neurosciences·Cynthia L Bethea, Arubala P Reddy
Apr 2, 2011·Respiratory Physiology & Neurobiology·Matthew R HodgesGeorge B Richerson
Dec 30, 2014·Progress in Neuro-psychopharmacology & Biological Psychiatry·Cynthia L BetheaJon E Levine
Jul 6, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Arubala P ReddyNurgul Carkaci-Salli

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