Effect of ovarian storage time at 4 degrees C on cumulus cell apoptosis in porcine antral follicles

Animal Science Journal = Nihon Chikusan Gakkaihō
Yafei ChiJing Lu

Abstract

The present study was conducted to investigate the effect of cold storage time on apoptosis of cumulus cells (CCs) from porcine ovaries, and to compare the sensitivity of four apoptosis-detection methods. Porcine ovaries were stored in physiological saline solution at 4°C for 0, 7, 24 and 48 hr, and then cumulus cells or granulosa cells (GCs) in antral follicles were retrieved to detect cell apoptosis. Cumulus cells isolated from stored ovaries for 24 hr presented obvious apoptosis using terminal deoxynucleotidyl transferase (TdT)-mediated d-UTP nick end-labeling (TUNEL) assay. A typical DNA ladder pattern of apoptosis was observed in GCs 24 hr post storage treatment. The mean Olive Tail Moment of CCs was significantly increased after 24 hr using comet assay; however, the mean tail migration and mean tail DNA increased gradually after 7 hr of storage. In addition, annexin V/PI staining assay showed an obvious increase in apoptotic CCs (Annexin V positive, PI negative) 7 hr after treatment, and the apoptotic rate reached to a peak at 24 hr followed by a decline after 48 hr of storage to the level at 7 hr. In conclusion, cold storage of porcine ovary in physiological saline solution induced a time-dependent increase in apoptosis ...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Feb 1, 1983·Biology of Reproduction·B D BavisterG Lieberman
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill
Aug 28, 1998·Science·G Evan, T Littlewood
Mar 21, 2000·Journal of Molecular and Cellular Cardiology·B FreudeJ Schaper
Jun 19, 2001·Mutation Research·P ChoucrounJ D Dewitte
Feb 9, 2002·Molecular Reproduction and Development·Sofie TangheAart de Kruif
May 7, 2002·Theriogenology·Margot Alves Nunes Dode, Charles Graves
Mar 5, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Abdulla K SalahudeenJohn K Jenkins
Mar 11, 2003·Zygote : the Biology of Gametes and Early Embryos·Hisataka IwataYoshiharu Nagai
Jun 5, 2003·Molecular Reproduction and Development·Mauro MattioliGioia Luisa
Jun 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Oliver DrognitzStefan Benz
Mar 8, 2005·The Journal of Reproduction and Development·Pimprapar WongsrikeaoTakashi Nagai
Dec 2, 2006·The Journal of Reproduction and Development·Hideaki NaoiTakashi Nagai

❮ Previous
Next ❯

Citations

Nov 17, 2021·Neural Regeneration Research·Ying-Jie ZhaoFang Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis