Effect of ovary holding temperature and time on equine granulosa cell apoptosis, oocyte chromatin configuration and cumulus morphology

Theriogenology
Hanne G PedersenEvelyn E Telfer

Abstract

The effects of ovary holding time and temperature on granulosa cell apoptosis, oocyte chromatin configuration and cumulus morphology were investigated through a series of experiments. Three experiments were performed to determine the effect of ovary holding time and temperature on granulosa cell apoptosis. Ovaries were held (1) at 20, 30 or 35-37 degrees C for up to 2h, (2) at 30 degrees C for 0-1, 1-2, 2-3, 3-4, 4-6 or 6-10h, and (3) granulosa cells were held for 0, 1, 2, 3, 5, 12 or 24h in M199 with Hank's salts at room temperature (suboptimal incubation). Granulosa cell DNA was analysed by ethidium bromide staining or 3'-end labelling. Two experiments were performed to determine the effect of ovary holding time and temperature on oocyte chromatin configuration. Ovaries were held (1) at 20, 30 or 35-37 degrees C for up to 3h and (2) at 20-37 degrees C for 0-1, 1-2, 2-3, 3-4, 4-6, 6-8 or 8-12h. The oocytes were stained with Hoechst stain 33258 and the chromatin configuration was evaluated. Two experiments were performed to determine the effect of ovary holding time and temperature on cumulus oophorus morphology. Ovaries were held at (1) 20-30 or 35-37 degrees C for up to 2h and (2) for 0-2, 2-4, 4-6, and 6-10h at 35-37 degrees...Continue Reading

References

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Citations

Mar 8, 2005·The Journal of Reproduction and Development·Pimprapar WongsrikeaoTakashi Nagai
Dec 2, 2006·The Journal of Reproduction and Development·Hideaki NaoiTakashi Nagai
Nov 21, 2008·Molecular Human Reproduction·Jing-He TanJie Zhang
Nov 30, 2006·The Veterinary Clinics of North America. Equine Practice·Elaine M Carnevale, Lisa J Maclellan
Aug 4, 2006·Microscopy Research and Technique·Yong LiuJing-He Tan
Sep 14, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert M Zucker, Susan C Jeffay
Jul 24, 2010·Molecular Reproduction and Development·Katrin Hinrichs
Mar 12, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·A BerteroT Nervo
Jul 23, 2020·Reproduction, Fertility, and Development·Elizabeth S MetcalfThomas O Leary
Nov 23, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Yafei ChiJing Lu
Dec 23, 2020·Animals : an Open Access Journal From MDPI·Alicia Martín-MaestroAna Josefa Soler
Dec 9, 2020·Reproductive Biomedicine Online·Janice de Miranda Vasconcellos VilelaChristiani Andrade Amorim
Jan 4, 2019·Environmental Pollution·Fei-Ping LiHai-Long Wang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis