Abstract
The effects of ovary holding time and temperature on granulosa cell apoptosis, oocyte chromatin configuration and cumulus morphology were investigated through a series of experiments. Three experiments were performed to determine the effect of ovary holding time and temperature on granulosa cell apoptosis. Ovaries were held (1) at 20, 30 or 35-37 degrees C for up to 2h, (2) at 30 degrees C for 0-1, 1-2, 2-3, 3-4, 4-6 or 6-10h, and (3) granulosa cells were held for 0, 1, 2, 3, 5, 12 or 24h in M199 with Hank's salts at room temperature (suboptimal incubation). Granulosa cell DNA was analysed by ethidium bromide staining or 3'-end labelling. Two experiments were performed to determine the effect of ovary holding time and temperature on oocyte chromatin configuration. Ovaries were held (1) at 20, 30 or 35-37 degrees C for up to 3h and (2) at 20-37 degrees C for 0-1, 1-2, 2-3, 3-4, 4-6, 6-8 or 8-12h. The oocytes were stained with Hoechst stain 33258 and the chromatin configuration was evaluated. Two experiments were performed to determine the effect of ovary holding time and temperature on cumulus oophorus morphology. Ovaries were held at (1) 20-30 or 35-37 degrees C for up to 2h and (2) for 0-2, 2-4, 4-6, and 6-10h at 35-37 degrees...Continue Reading
References
May 1, 1991·The Journal of Experimental Zoology·A C SchroederJ J Eppig
Nov 1, 1985·Journal of Reproduction and Fertility·R M Moor, I M Crosby
Apr 10, 1980·Nature·A H Wyllie
Mar 1, 1993·Journal of Cellular Physiology·J L Tilly, A J Hsueh
Feb 1, 1993·Biology of Reproduction·K HinrichsM G Martin
Nov 1, 1996·Biology of Reproduction·R NahumA Tsafriri
Aug 1, 1997·Biology of Reproduction·K Hinrichs, K A Williams
Oct 10, 1997·Molecular Reproduction and Development·K JewgenowD E Wildt
Sep 1, 1996·Reviews of Reproduction·J L Tilly
Mar 29, 2000·Theriogenology·F GuignotE Palmer
Apr 25, 2000·Biology of Reproduction·K Hinrichs, A L Schmidt
Jun 18, 2002·Theriogenology·C C LoveK Hinrichs
Jan 9, 2003·Theriogenology·Linda B LoveKatrin Hinrichs
Feb 28, 2003·Biology of Reproduction·Maria Elena Dell'AquilaKatrin Hinrichs
Mar 4, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·H G PedersenE E Telfer
Oct 1, 2003·The Veterinary Record·S Sirisathien, B G Brackett
May 1, 1995·Theriogenology·M R Del CampoO J Ginther
Nov 1, 1996·Theriogenology·F J Richard, M A Sirard
Apr 1, 1997·Theriogenology·P BlondinM A Sirard
Citations
Dec 2, 2006·Reproductive Biology and Endocrinology : RB&E·Fiorenza MinerviniAngelo Visconti
Mar 8, 2005·The Journal of Reproduction and Development·Pimprapar WongsrikeaoTakashi Nagai
Dec 2, 2006·The Journal of Reproduction and Development·Hideaki NaoiTakashi Nagai
Jun 26, 2008·Reproduction, Fertility, and Development·R N ChavesJ R Figueiredo
Jan 14, 2005·Biology of Reproduction·K HinrichsB E Walckenaer
Nov 21, 2008·Molecular Human Reproduction·Jing-He TanJie Zhang
Feb 22, 2011·Animal Reproduction Science·Y S WangY Zhang
Nov 30, 2006·The Veterinary Clinics of North America. Equine Practice·Elaine M Carnevale, Lisa J Maclellan
Aug 4, 2006·Microscopy Research and Technique·Yong LiuJing-He Tan
Sep 14, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert M Zucker, Susan C Jeffay
Jul 24, 2010·Molecular Reproduction and Development·Katrin Hinrichs
Apr 30, 2013·Theriogenology·Andreas VernunftHelmut Torner
Jun 21, 2016·Scientific Reports·Juan LinJing-He Tan
Sep 8, 2012·Biology of Reproduction·Qing LiJing-He Tan
Mar 12, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·A BerteroT Nervo
Mar 26, 2014·Journal of Animal Science·A GambiniD F Salamone
Jul 23, 2020·Reproduction, Fertility, and Development·Elizabeth S MetcalfThomas O Leary
Oct 22, 2019·Reproduction, Fertility, and Development·Valentina LoddeFederica Franciosi
Feb 1, 2008·Reproductive Medicine and Biology·Misa HosoeTomoyuki Tokunaga
Nov 23, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Yafei ChiJing Lu
Dec 23, 2020·Animals : an Open Access Journal From MDPI·Alicia Martín-MaestroAna Josefa Soler
Dec 9, 2020·Reproductive Biomedicine Online·Janice de Miranda Vasconcellos VilelaChristiani Andrade Amorim
Jan 4, 2019·Environmental Pollution·Fei-Ping LiHai-Long Wang