Effect of overexpressing rsmA from Pseudomonas aeruginosa on virulence of select phytotoxin-producing strains of P. syringae

Phytopathology
Hye Suk KongJohn Lydon

Abstract

The GacS/GacA two-component system functions mechanistically in conjunction with global post-transcriptional regulators of the RsmA family to allow pseudomonads and other bacteria to adapt to changing environmental stimuli. Analysis of this Gac/Rsm signal transduction pathway in phytotoxin-producing pathovars of Pseudmonas syringae is incomplete, particularly with regard to rsmA. Our approach in studying it was to overexpress rsmA in P. syringae strains through introduction of pSK61, a plasmid constitutively expressing this gene. Disease and colonization of plant leaf tissue were consistently diminished in all P. syringae strains tested (pv. phaseolicola NPS3121, pv. syringae B728a, and BR2R) when harboring pSK61 relative to these isolates harboring the empty vector pME6031. Phaseolotoxin, syringomycin, and tabtoxin were not produced in any of these strains when transformed with pSK61. Production of protease and pyoverdin as well as swarming were also diminished in all of these strains when harboring pSK61. In contrast, alginate production, biofilm formation, and the hypersensitive response were diminished in some but not all of these isolates under the same growth conditions. These results indicate that rsmA is consistently im...Continue Reading

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Citations

Apr 3, 2013·Antimicrobial Agents and Chemotherapy·Mai AlhajlanAbdelwahab Omri
Apr 17, 2013·BMC Microbiology·Jackeline Lizzeta Arvizu-GómezAriel Álvarez-Morales
Jun 26, 2013·Environmental Microbiology·Emanuela FrangipaniFrancesco Imperi
Nov 20, 2013·Environmental Microbiology Reports·Paola VargasMaría-Trinidad Gallegos
Jun 14, 2014·FEMS Microbiology Reviews·María A LlamasIain L Lamont
Dec 21, 2017·Molecular Plant-microbe Interactions : MPMI·María-Dolores FerreiroMaría Trinidad Gallegos
Apr 3, 2015·Microbiology and Molecular Biology Reviews : MMBR·Christopher A VakulskasTony Romeo
Apr 14, 2015·The New Phytologist·Lamia HarfoucheWafa Achouak
May 4, 2021·Environmental Microbiology·María-Dolores Ferreiro, María-Trinidad Gallegos
Jun 19, 2021·Journal of Microbiological Methods·Jeffrey K Schachterle, Qi Huang

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