Effect of oxidative stress on in vivo ADP-ribosylation of eukaryotic elongation factor 2

The International Journal of Biochemistry & Cell Biology
M BektaşE Bermek

Abstract

Different lines of evidence indicate that eukaryotic elongation factor 2 (eEF2) can be ADP-ribosylated endogenously. The physiological significance of this reaction has, however, remained unclarified. In order to address this issue we investigated the in vivo ADP-ribosylation of eEF2 and the effect of oxidative stress thereon. The investigation revealed that the endogenous ADP-ribosylation of eEF2 is complex and can take place in K562 cell lysates either under the action of endogenous transferase from [adenosine-14C]NAD or by direct binding of free [14C]ADP-ribose. These two types of ADP-ribosylation were distinguished by use of different treatments based on the chemical stability of the respective bonds formed. Under standard culture conditions, in vivo labeling of eEF2 in the presence of [14C]adenosine was reversed to about 65% in the presence of diphtheria toxin and nicotinamide. This finding implied that the modification that took place under physiological circumstances was, mainly, of an enzymic nature. On the other hand, H2O2-promoted oxidative stress gave rise to a nearly two-fold increase in the extent of in vivo labeling of eEF2. This was accompanied by a loss of eEF2 activity in polypeptide chain elongation. Oxidative...Continue Reading

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Citations

Nov 28, 2012·PloS One·Ricardo A GomesCarlos Cordeiro
Jun 15, 2011·The International Journal of Biochemistry & Cell Biology·Muhammet BektaşEngin Bermek
Mar 21, 2008·Cytokine & Growth Factor Reviews·Shai White-GilbertsonChristina Voelkel-Johnson
Aug 28, 2009·Cell Biochemistry and Function·Muhammet BektaşEngin Bermek
Sep 19, 2008·The Plant Journal : for Cell and Molecular Biology·Takahisa OgawaShigeru Shigeoka
Mar 1, 2006·Insect Biochemistry and Molecular Biology·Frédéric FrancisEric Haubruge
Feb 14, 2006·Biochimica Et Biophysica Acta·Sandro ArgüellesAntonio Ayala
Dec 9, 2014·Bioscience, Biotechnology, and Biochemistry·Kazuya Yoshimura, Shigeru Shigeoka
Nov 8, 2014·Bioscience, Biotechnology, and Biochemistry·Sayuri TanakaManabu Sugimoto
Sep 12, 2018·Journal of Biochemistry·Kayoko Tsuda-Sakurai, Masayuki Miura
Nov 21, 2007·Organic & Biomolecular Chemistry·Hening Lin
Sep 6, 2005·Cell Biochemistry and Function·Muhammet BektaşEngin Bermek
Sep 17, 2016·Cell Cycle·Iok In Christine Chio, David A Tuveson

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