Effect of oxygen free radicals on myosin in muscle fibres

FEBS Letters
F KönczölJ Belágyi

Abstract

Experiments were performed on glycerol-extracted muscle fibres prepared from psoas muscle of rabbit in the presence of hydroxyl free radical generating system. Short irradiation of spin-labelled muscle fibres by UV light showed the interaction of probe molecules with oxygen free radicals. The intensity of the EPR signal from maleimide or isothiocyanate spin labels attached to the essential thiol groups decreased following irradiation. Oxygen free radicals affected the rate constant of the transition AM.ADP.Vi-->AM.ADP in the ATP hydrolysis cycle. It was found that the essential -SH groups of myosin were involved in the oxidation of sulphydryls by Ce(IV). Ce(IV) complexed to nitrilotriacetic acid in the presence of spin trap produced long-lived free radicals located partly on SH-1 sulphydryls.

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Citations

Oct 11, 2008·Pflügers Archiv : European journal of physiology·Stéphane DelliauxYves Jammes
Dec 21, 2004·Redox Report : Communications in Free Radical Research·Tet Fatt ChiaLian Sai Chia
Jan 12, 2008·Journal of Applied Physiology·Brian J HardinMichael B Reid
Nov 28, 2007·The Biochemical Journal·Marianne N LundMichael J Davies
Nov 17, 2007·Journal of Applied Physiology·Leonardo F Ferreira, Michael B Reid
May 2, 2009·Journal of Applied Physiology·Leonardo F FerreiraMichael B Reid
Apr 9, 2008·Journal of Agricultural and Food Chemistry·Anne M FrederiksenLeif H Skibsted

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