Effect of oxygen on cyclic GMP-dependent protein kinase-mediated relaxation in ovine fetal pulmonary arteries and veins

American Journal of Physiology. Lung Cellular and Molecular Physiology
Yuansheng GaoJ U Raj

Abstract

Cyclic GMP-dependent protein kinase (PKG) plays an important role in regulating pulmonary vasomotor tone in the perinatal period. In this study, we tested the hypothesis that a change in oxygen tension affects PKG-mediated pulmonary vasodilation. Isolated intrapulmonary arteries and veins of near-term fetal lambs were first incubated for 4 h under hypoxic and normoxic conditions (Po2 of 30 and 140 mmHg, respectively) and then contracted with endothelin-1. 8-Bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), a cell membrane-permeable analog of cGMP, induced a greater relaxation in vessels incubated in normoxia than in hypoxia. beta-Phenyl-1,N2-etheno-8-bromoguanosine-3',5'-cyclic monophosphorothioate, Rp isomer (Rp-8-Br-PET-cGMPS), a selective inhibitor of PKG, attenuated relaxation induced by 8-BrcGMP (10-4 and 3 x 10-4 M). In the presence of Rp-8-Br-PET-cGMPS, the differential responses to 8-BrcGMP between hypoxia and normoxia treatment were abolished in veins but not in arteries. cGMP-stimulated PKG activity was present in arteries but not in veins after 4 h of hypoxia. Both vessel types showed significant increase in cGMP-stimulated PKG activity after 4 h of normoxia. PKG protein (Western blot analysis) and PKG mRNA level...Continue Reading

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Citations

Sep 2, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yuansheng GaoJ Usha Raj
Oct 28, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·William J PerkinsKeith A Jones
Jan 13, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jie LiuJ Usha Raj
May 5, 2009·American Journal of Physiology. Heart and Circulatory Physiology·S NegashJ Usha Raj
Dec 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patricia R BachillerJesse D Roberts
Mar 1, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jie LiuVirender K Rehan
Oct 15, 2011·American Journal of Respiratory Cell and Molecular Biology·Ramaswamy RamchandranJ Usha Raj
Nov 30, 2011·Circulation Journal : Official Journal of the Japanese Circulation Society·Dou DouYuansheng Gao
May 15, 2004·American Journal of Respiratory and Critical Care Medicine·Tamás DolinayAugustine M K Choi
Feb 26, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nandy C LopezAnibal J Llanos
Oct 21, 2010·Physiological Reviews·Yuansheng Gao, J Usha Raj
Aug 6, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Virender K RehanJohn S Torday

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