Effect of P2 receptor on the intracellular calcium increase by cancer cells in human umbilical vein endothelial cells.

Naunyn-Schmiedeberg's Archives of Pharmacology
Namie NejimeKazumasa Shinozuka

Abstract

One of the important functions of vascular endothelial cells is as a barrier between blood and vascular tissue. This led us to speculate that cancer cells affect endothelial cells during metastasis. In the present study, we investigated the influence of human fibrosarcoma cells (HT-1080) on human umbilical vein endothelial cells (HUVEC), particularly intracellular calcium ion levels ([Ca2+]i), which are known to be an important intracellular signal transduction factor. HUVEC were treated with a fluorescent marker, and the fluorescence intensity of [Ca2+]i was then measured by phase contrast microscopic imaging. Extracellular adenosine triphosphate (ATP) release was measured using the chemiluminescence of luciferin-luciferase and a photon counting imaging system. HT-1080 (5x10(4) cells per dish) was found to increase [Ca2+]i in HUVEC. This [Ca2+]i rise was significantly reduced by U-73122 (phospholipase C inhibitor, 1 microM) and thapsigargin (calcium pump inhibitor, 1 microM). Interestingly, the [Ca2+]i rise in HUVEC was also significantly reduced by pyridoxalphosphare-6-azophenyl-2', 4'-disulfonic acid, a P2Y receptor antagonist (100 microM) and apyrase, a nucleotidase inhibitor (2 U/ml). In addition, we observed ATP release f...Continue Reading

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Citations

Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Nov 7, 2008·Clinical and Experimental Pharmacology & Physiology·Namie NejimeKazumasa Shinozuka
Jan 14, 2010·Science Signaling·Ross Corriden, Paul A Insel
Aug 21, 2012·World Journal of Biological Chemistry·Francesco MocciaFranco Tanzi
Jan 20, 2010·Biochimica Et Biophysica Acta·Stephan LorenzKnut Krohn
Jun 13, 2014·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Kazumasa ShinozukaKazuki Nakamura
Jan 18, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Alaa E El-SisiSally E Abu-Risha

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