PMID: 25773816Mar 17, 2015Paper

Effect of paclitaxel-loaded nanoparticles on the viability of human hepatocellular carcinoma HepG2 cells

Asian Pacific Journal of Cancer Prevention : APJCP
Zhi-Hong HouWei Zheng

Abstract

To explore effects of paclitaxel-loaded poly lactic-co-glycolic acid (PLGA) particles on the viability of human hepatocellular carcinoma (HCC) HepG2 cells. The viability of HepG2 cells was assessed using MTT under different concentrations of prepared paclitaxel-loaded particles and paclitaxel (6.25, 12.5, 25, 50, and 100 mg/L), and apoptosis was analyzed using Hochest33342/Annexin V-FITC/PI combined with an IN Cell Analyzer 2000. Paxlitaxel-loaded nanoparticles were characterized by narrow particle size distribution (158.6 nm average particle size). The survival rate of HepG2 cells exposed to paclitaxel-loaded PLGA particles decreased with the increase of concentration and time period (P<0.01 or P<0.05), the dose- and time-dependence indicating sustained release (P<0.05). Moreover, apoptosis of HepG2 cells was induced, again with an obvious dose- and time-effect relationship (P<0.05). Paclitaxel-loaded PLGA particles can inhibit the proliferation and induce the apoptosis of HCC HepG2 cells. This new-type of paclitaxel carrier body is easily made and has low cost, good nanoparticle characterization and sustained release. Hence, paclitaxel- loaded PLGA particles deserve to be widely popularized in the clinic.

References

Jan 24, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·X L DongT Wang
Jun 4, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Supakorn PitakkarnkulThaovalai Thavaramara
Jan 13, 2015·International Journal of Pharmaceutics·Fabienne DanhierVéronique Préat

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Citations

Nov 4, 2015·Acta Biochimica Et Biophysica Sinica·Guojin HuangJianqiang Zhao
Jul 26, 2019·Journal of Experimental & Clinical Cancer Research : CR·Kai TengDan Xie

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