Effect of pancreatic warm ischemia on islet yield and viability in dogs

Transplantation
Y TaniokaJ P Leone

Abstract

Defining tolerable warm ischemia (WI) is mandatory before nonheartbeating cadavers can be used to enlarge the donor pool. No studies to date have precisely evaluated the effect of pancreatic WI on islet yield and viability in a large animal model. We used mongrel dogs in our study at the University of Minnesota. Excised pancreases were left in situ for a designated period (0, 30, 45, and 60 min in groups 1 to 4, respectively) of WI. Then, they were digested by the automated collagenase digestion method of Ricordi, purified on Euro-Ficoll discontinuous gradients with the COBE cell processor, and autotransplanted into the liver via a mesenteric vein. We compared the four groups in terms of islet yield, expressed as islet equivalents (IE; diameter standardizing to 150 microm) per pancreas weight (IE/g pancreas), and viability, assessed by functional success (maintenance of normoglycemia for 2 weeks) after transplant. Mean islet yield (+/- SD) and the functional success rate after transplant were as follows: 6200+/-1800 IE/g pancreas and 4 of 4 (100%) in group 1; 6300+/-4400 and 4 of 4 (100%) in group 2; 3800+/-2600 and 2 of 4 (50%) in group 3; and 1400+/-1300 and 0 of 4 (0%) in group 4 (P=0.01 vs. group 1). With 30 min or less of ...Continue Reading

References

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Citations

Jul 5, 2005·Journal of Hepato-biliary-pancreatic Surgery·Shinichi Matsumoto, Koichi Tanaka
Jun 7, 2003·Transplantation·James F MarkmannAli Naji
Jul 28, 2011·Pancreas·Orison O WoolcottRobert H Chow
Sep 3, 2010·Transplantation·Takuro SaitoUNKNOWN Working Members of The Japanese Pancreas and Islet Transplantation Association
Jul 9, 2010·World Journal of Gastroenterology : WJG·Yasuhiro Fujino
Apr 26, 2006·Clinical and Experimental Immunology·T LinnD Brandhorst
Jul 22, 2014·Xenotransplantation·Bradley P WeegmanKlearchos K Papas
Mar 19, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Mettu S ReddyDavid Talbot
Nov 22, 2005·Transplantation Proceedings·T SaitoS Teraoka
Apr 15, 2015·Biochimica Et Biophysica Acta·Sandrine LablancheEric Fontaine
Nov 18, 2003·Immunological Reviews·A M James ShapiroJonathan R T Lakey
Jul 17, 2012·Cell Transplantation·Bashoo NaziruddinMarlon F Levy
Dec 5, 2020·Transplant International : Official Journal of the European Society for Organ Transplantation·Thomas PrudhommeJulien Branchereau
Feb 26, 2021·The Journal of International Medical Research·Shengmin MeiZhenhua Hu

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