Effect of parental growth on dynamics of conjugative plasmid transfer in the pea spermosphere.

Applied and Environmental Microbiology
P Sudarshana, G R Knudsen

Abstract

Plasmid transfer rates for the conjugative plasmid R388::Tn1721 from Pseudomonas cepacia (donor) to Pseudomonas fluorescens (recipient) on agar media, in broth, and in microcosms containing sterile or nonsterile soil, in the presence or absence of germinating pea seeds, were determined. Donors, recipients, and transconjugants were enumerated on selective media after 1 day on agar or in broth culture and over a 7-day period in soil or pea spermosphere microcosms. Donor and recipient growth rates and plasmid transfer rate constants [(gamma), where (gamma) = transconjugants (middot) (donors (middot) recipients)(sup-1) (middot) h(sup-1)] were calculated for three initial parental densities (10(sup4), 10(sup6), or 10(sup8) CFU/g or ml) in each system. For all initial density levels, values of (gamma) in agar and broth matings were higher than those in soil or in the pea spermosphere-rhizosphere microcosms. Values of (gamma) were not influenced by the pea spermosphere or by sterile or nonsterile conditions of the soil. However, (gamma) values in microcosm experiments were inversely related to initial parental density and were directly related to donor growth rates. Values of (gamma) averaged 4 x 10(sup-10), 4 x 10(sup-12), and 3 x 10...Continue Reading

References

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Nov 1, 1991·Applied and Environmental Microbiology·B K Kinkle, E L Schmidt

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Citations

Jul 31, 2004·Annual Review of Phytopathology·Eric B Nelson
Apr 3, 2009·The ISME Journal·Rembrandt J F HaftBeth Traxler
May 30, 2006·Current Microbiology·Padma Sudarshana, Guy R Knudsen
Jul 6, 2000·Applied and Environmental Microbiology·A M HohnstockE L Madsen

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