PMID: 22586914May 17, 2012Paper

Effect of partial pressure of CO2 on the production of thermostable alpha-amylase and neutral protease by Bacillus caldolyticus

Prikladnaia biokhimiia i mikrobiologiia
J BaderR Bajpai

Abstract

Controlling the concentration of dissolved oxygen is a standard feature in aerobic fermentation processes but the measurement of dissolved CO2 concentrations is often neglected in spite of its influence on the cellular metabolism. In this work room air and room air supplemented with 5% and 10% carbon dioxide were used for aeration during the cultivation of the thermophilic microorganism Bacillus caldolyticus (DSM 405) on starch to produce alpha-amylase (E.C. 3.2.1.1) and neutral protease (E.C. 3.4.24.27/28). The increased CO2 concentrations resulted in a 22% raise in activity of secreted alpha-amylase and a 43% raise in protease activity when compared with aeration with un-supplemented room air. There was no effect on the final biomass concentration. Furthermore, the lag-phase of fermentation was reduced by 30%, further increasing the productivity of alpha-amylase production. Determinations of dissolved CO2 in the culture broth were conducted both in situ with a probe as well as using exhaust gas analysis and both the methods of quantification showed good qualitative congruence.

References

Oct 1, 1969·Canadian Journal of Microbiology·J E ZajicA Wellman
Jul 21, 2005·Journal of Bacteriology·Melissa DrysdaleTheresa M Koehler

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Citations

Apr 30, 2014·Biotechnology Progress·Marlene LopesManuel Mota
Jan 19, 2013·Bulletin of Experimental Biology and Medicine·K P Ivanov, N N Mel'nikova
Aug 5, 2017·Journal of Industrial Microbiology & Biotechnology·Brady D LeePeter P Sheridan

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