Effect of patient, operative and isolation factors on subsequent yield and viability of human hepatocytes for research use

Cell and Tissue Banking
Tom LloydAshley Dennison

Abstract

It is widely accepted that the model of choice for pharmacotoxicological studies are human hepatocytes. There is therefore a demand for these cells, but quality must be maintained for their widespread use. We present a retrospective review of the isolation of hepatocytes from both surgically resected tissue and livers rejected for transplantation, and evaluated patient, operative and isolation variables to ascertain which may affect the viability and yield of cells. Seven clinically rejected whole livers and 60 surgically resected specimens (from two distinct operating centres) were isolated. For surgically resected tissue we found that decreasing age, securing the perfusing cannulae with suture rather than reforming Glissons capsule with glue and steatotic livers improved viability. No significant correlation could be found with pre-operative blood results, disease, type of operation, presence or absence of Pringle manoeuvre, weight of tissue isolated, time of digestion with collagenase and cold ischaemic time. There was a reduction in mean yield and viability when hepatocyte isolations were performed in livers rejected for transplant, compared to surgically resected tissue although this did not reach significance. Human hepat...Continue Reading

Citations

Oct 10, 2015·Tissue Engineering. Part C, Methods·Martin KlugeNathanael Raschzok
Jun 20, 2007·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·J AkhterD L Morris
Jan 22, 2014·Cytotechnology·Xiaobing GongPeter Neuhaus
Oct 20, 2005·Journal of Bioscience and Bioengineering·Jung-Keug Park, Doo-Hoon Lee
Feb 3, 2009·Cell Transplantation·María Teresa DonatoMaría José Gómez-Lechón
Feb 1, 2018·Tissue Engineering. Part C, Methods·Rebeka D MajorNathanael Raschzok
Oct 1, 2016·World Journal of Gastroenterology : WJG·Fan-Ying MengPing Zhou
May 8, 2018·Current Transplantation Reports·James E SquiresAlejandro Soto-Gutierrez
Jan 2, 2018·Experimental Cell Research·Colin H BeckwittAlan Wells

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