Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742

Bioresource Technology
Azis Boing SitanggangYi-Cheng Ho

Abstract

The higher GlcN production using a wild-type fungi, Aspergillus sp. BCRC 31742 cultivated under submerged fermentation was investigated. Several fermentation aspects were studied, such as pellet size, working volume, agitation rate and stimulating factor. Culture cultivation with conditions, such as pellet diameter of 2.15mm, 50mL working volume (250mL T-flask), incubation at 30 degrees C, 200rpm and pH 7.0 for 5days yielded highest biomass concentration which was 33.82g/L, with a GlcN concentration of 7.05g/L. Methanol was found to give the best stimulatory effect in terms of GlcN concentration as compared to glutamic acid, cycloheximide and ethanol. Addition of methanol (1.5%v/v) into fermentation medium could increase GlcN content from 0.21 (control) to 0.26g/gdw cells and led to maximum GlcN concentration of 7.48g/L obtained.

Citations

Jun 12, 2013·Applied Microbiology and Biotechnology·Long LiuJian Chen
Aug 22, 2013·Applied Biochemistry and Biotechnology·Marzieh MohammadiKeikhosro Karimi
Oct 17, 2014·Indian Journal of Microbiology·Sheng WangXiangkun Wu
Jul 3, 2013·International Journal of Biological Macromolecules·Yuying Sun Shujun Wang
Sep 19, 2015·Critical Reviews in Biotechnology·Jianguo Zhang, Jining Zhang
Jan 20, 2016·Applied Microbiology and Biotechnology·Gazi Sakir HossainLong Liu
Mar 13, 2016·Bioprocess and Biosystems Engineering·Song-Fang HanZhi-Han Wang
Sep 6, 2012·Biotechnology and Bioengineering·David J Barry
Jan 18, 2018·The Journal of Biological Chemistry·Lisa A AndersonKristala L J Prather
Aug 24, 2019·Applied Microbiology and Biotechnology·Qinyuan Ma, Xiuzhen Gao
Jun 21, 2020·International Journal of Molecular Sciences·Adriana Ferreira de SouzaGalba Maria de Campos-Takaki
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jia Wei Peng, Ho Shing Wu
Apr 3, 2021·Environmental Science and Pollution Research International·Ayusmita RayDebabrata Das
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valerio ZulloLorenzo Guazzelli
Jun 18, 2019··Zhao Wen, Zhao Wen

❮ Previous
Next ❯

Related Concepts

Related Feeds