Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222

Letters in Applied Microbiology
M SunairiM Nakajima

Abstract

Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different transfection frequencies were observed. Penicillin G enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants.

References

May 1, 1990·Trends in Biochemical Sciences·M Solioz, D Bienz
Apr 1, 1988·Biochimie·A Mercenier, B M Chassy
Feb 1, 1974·Journal of General Microbiology·M OkanishiH Umezawa
Jan 1, 1993·Applied and Environmental Microbiology·M SunairiM Nakajima

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