Effect of Period 2 on the proliferation, apoptosis and migration of osteosarcoma cells, and the corresponding mechanisms

Oncology Letters
Tao QinYuan-Yuan Sun

Abstract

Period 2 (per2) is a core circadian clock gene. Dysregulation of the per2 gene has been identified in a number of types of human cancer and may be associated with a poor prognosis. To confirm the influence of per2 gene on MNNG/HOS human osteosarcoma cells, small interfering (si)RNA against per2 or plasmids containing per2 were transfected into MNNG/HOS cells, and the proliferation, apoptosis and migration were observed. The present study demonstrated that per2 knockdown significantly enhanced MNNG/HOS cell proliferation and migration and protected MNNG/HOS cells from apoptosis. Per2 overexpression inhibited MNNG/HOS cell proliferation and migration and promoted apoptosis. Furthermore, the protein expression of phosphorylated (p)-protein kinase B (Akt) and Bcl-2 were inhibited in per2-overexpressing cells, while the expression of p27, p21 and cleaved caspase-3 was promoted. In contrast, the expression of p-Akt and Bcl-2 was promoted in per2-knockdown cells, and p27, p21 and cleaved caspase-3 were decreased. This initial study may provide an alternative therapeutic strategy for the treatment of osteosarcoma.

References

Nov 21, 1985·The New England Journal of Medicine·J E MullerT Robertson
Aug 1, 1995·Stroke; a Journal of Cerebral Circulation·M Kelly-HayesR B D'Agostino
Apr 23, 2002·Nature·Kai-Florian StorchCharles J Weitz
Aug 20, 2003·Archives of Otolaryngology--head & Neck Surgery·Rajesh V LallaDonald L Kreutzer
Jun 3, 2004·Cancer Research·Rebecca L ElstromCraig B Thompson
Mar 26, 2005·Carcinogenesis·Shou-Tung ChenJan-Gowth Chang
Jul 11, 2006·Cancer Science·Hui HuaFranz Halberg
Apr 30, 2008·Molecular Carcinogenesis·Yueh-Min LinJan-Gowth Chang
Jul 25, 2008·Breast Cancer Research and Treatment·Xiaoming YangWilliam J M Hrushesky
Jan 31, 2009·Australian Veterinary Journal·C PepplerR Neiger
Jun 2, 2009·Neoplasia : an International Journal for Oncology Research·Kathleen G NeivaJacques E Nör
Mar 10, 2010·Cancer Treatment and Research·Giulia Ottaviani, Norman Jaffe
Mar 20, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koyomi MiyazakiNorio Ishida
Jan 11, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Hans GelderblomUNKNOWN European Osteosarcoma Intergroup
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Apr 21, 2011·Expert Review of Anticancer Therapy·George SgourakisHauke Lang
Sep 14, 2011·International Journal of Cancer. Journal International Du Cancer·Jeremy WhelanSandra J Strauss
Oct 13, 2011·The Journal of Pathology·Gareth H Williams, Kai Stoeber
Jan 19, 2012·Cancer Letters·Michael W Greene
Jun 12, 2012·Sarcoma·Jeff W MartinMaria Zielenska
Aug 11, 2012·Progress in Brain Research·Jürgen A Ripperger, Urs Albrecht
Aug 22, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xiaoming YangEsmaiel Jabbari
Dec 21, 2012·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Daniel RellesHwyda A Arafat
May 15, 2013·Current Opinion in Oncology·Jilong Yang, Wei Zhang
Aug 7, 2013·Trends in Cell Biology·Carrie L PartchJoseph S Takahashi
Oct 9, 2013·Cancer Letters·Fergal C KelleherAnne Maguire
Mar 4, 2014·World Journal of Gastroenterology : WJG·Theodoros KarantanosMaria Gazouli
Feb 19, 2016·Occupational and Environmental Medicine·Anne B HansenZorana J Andersen
Mar 30, 2018·DNA Repair·Eloy Andrés Pérez-YépezLuis Enrique Arias-Romero

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Feb 6, 2020·International Journal of Molecular Sciences·Ericka J D SilveiraCristiane H Squarize

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