Effect of Permeation Enhancers on the Buccal Permeability of Nicotine: Ex vivo Transport Studies Complemented by MALDI MS Imaging

Pharmaceutical Research
Eva MarxenJoseph A Nicolazzo

Abstract

The purpose of this study was to assess the effect of several chemical permeation enhancers on the buccal permeability of nicotine and to image the spatial distribution of nicotine in buccal mucosa with and without buccal permeation enhancers. The impact of sodium taurodeoxycholate (STDC), sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and Azone® on the permeability of [3H]-nicotine and [14C]-mannitol (a paracellular marker) across porcine buccal mucosa was studied ex vivo in modified Ussing chambers. The distribution of nicotine, mannitol and permeation enhancers was imaged using using matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI). Despite STDC significantly increasing permeability of [14C]-mannitol, no enhancing effect was seen on [3H]-nicotine permeability with any of the permeation enhancers. Rather, SDS and DMSO retarded nicotine permeability, likely due to nicotine being retained in the donor compartment. The permeability results were complemented by the spatial distribution of nicotine and mannitol determined with MALDI MSI. The buccal permeability of nicotine was affected in an enhancer specific manner, suggesting that nicotine primarily diffuses via the transcellular pathwa...Continue Reading

References

Jun 1, 1978·Journal of Pharmaceutical Sciences·V Shahi, J L Zatz
Jun 1, 1997·Journal of Pharmaceutical Sciences·A W LarhedE Björk
Jul 30, 1999·International Journal of Pharmaceutics·L L ChenY W Chien
Jul 20, 2002·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hanne Mørck Nielsen, Margrethe Rømer Rassing
Nov 7, 2003·Journal of Pharmaceutical Sciences·Joseph A NicolazzoBarrie C Finnin
Jan 6, 2004·Journal of Pharmaceutical Sciences·Joseph A NicolazzoBarrie C Finnin
Jul 6, 2004·Journal of Pharmaceutical Sciences·Joseph A NicolazzoBarrie C Finnin
May 17, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joseph A NicolazzoBarrie C Finnin
Nov 22, 2005·Expert Opinion on Drug Delivery·John D Smart
Jul 31, 2007·The Journal of Physical Chemistry. B·Andrey A Gurtovenko, Jamshed Anwar
Mar 20, 2010·AAPS PharmSciTech·Supriya S ShidhayeVilasrao J Kadam
Sep 17, 2010·Journal of Pharmaceutical Sciences·Lars B HeemstraJoseph A Nicolazzo
Nov 9, 2010·International Journal of Pharmaceutics·Longsheng HuBozena B Michniak-Kohn
Jun 21, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Dong-Ho OhSangkil Lee
Oct 4, 2011·International Journal of Pharmaceutics·Longsheng HuBozena B Michniak-Kohn
Mar 6, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian JanfeltHarald Severin Hansen
Mar 29, 2013·Journal of the American Society for Mass Spectrometry·Guillaume RobichaudDavid C Muddiman
May 9, 2013·Histochemistry and Cell Biology·Andreas Römpp, Bernhard Spengler
Feb 7, 2014·Drug Development and Industrial Pharmacy·Sanjeev RambharoseThirumala Govender
Feb 18, 2014·Journal of Pharmaceutical Sciences·Emil Meng-LundJoseph A Nicolazzo
Apr 2, 2014·Journal of Pharmaceutical Sciences·Thiago CaonJoseph A Nicolazzo
May 16, 2014·Journal of Pharmaceutical Sciences·Emil Meng-LundJette Jacobsen
Dec 9, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Liang JinJoseph A Nicolazzo
Jun 18, 2016·Journal of Pharmaceutical Sciences·Liang JinJoseph A Nicolazzo
Jul 19, 2016·International Journal of Pharmaceutics·Eva MarxenJette Jacobsen
Dec 10, 2016·The Journal of Physical Chemistry. B·Sabyasachi Chatterjee, Gopinatha Suresh Kumar
May 20, 2017·Methods in Molecular Biology·Paul J Trim

❮ Previous
Next ❯

Software Mentioned

GraphPad Prism
MSiReader
GraphPad

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.