PMID: 7543922Aug 1, 1995Paper

Effect of PGE2 and of agents that raise cAMP levels on macrophage activation induced by IFN-gamma and TNF-alpha

Journal of Leukocyte Biology
J MauëlY Buchmüller-Rouiller

Abstract

The effect of prostaglandin (PG) E2 on macrophage activation by interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) was evaluated. Murine macrophages infected with Leishmania enriettii or Leishmania major were activated by exposure to IFN-gamma (10-50 U/ml) and TNF-alpha (30-3000 U/ml), leading to intracellular parasite destruction within 24-48 h. Leishmanicidal activity was markedly increased when activation was performed in the presence of PGE2 (10(-9)-10(-7) M) or arachidonate (10(-5) M, a PG precursor), concomitant with enhanced nitrite release and glucose oxidation through the hexose monophosphate shunt pathway. Conversely, activation was reduced by indomethacin and hydrocortisone, two inhibitors of PG synthesis. Parasite killing and nitrite production were fully restored by exogenous PGE2, indicating that inhibition by these drugs was related to their ability to block PG production. PG can stimulate adenylate cyclase, thus raising intracellular cAMP levels. Accordingly, dibutyryl-cAMP, theophylline (which prevents cAMP breakdown), and forskolin (an activator of adenylate cyclase) all stimulated macrophage activation. Finally, PGE2 and cAMP enhanced expression of inducible nitric oxide synthase mRNA in...Continue Reading

Citations

Feb 24, 2001·Antioxidants & Redox Signaling·A Okado-MatsumotoN Taniguchi
Jan 24, 2004·Leukemia & Lymphoma·Arjen-Kars BoerEdo Vellenga
Sep 29, 2011·Immunology and Cell Biology·Vinatha SreeramkumarNatalia Cuesta
Jul 23, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amrita SahaAnindita Ukil
Dec 16, 2016·Journal of Neurochemistry·Takayuki NaganoMotohiko Takemura
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E Galea, D L Feinstein
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaocui ZhuSangdun Choi
Oct 30, 2007·The Journal of Pharmacology and Experimental Therapeutics·Sergey RyzhovIgor Feoktistov
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R IkegamiA Ichikawa
Apr 17, 1998·Immunopharmacology and Immunotoxicology·M A PanaroO Brandonisio
Dec 31, 1997·The American Journal of Physiology·S S GreenbergJ Ouyang
Apr 16, 2010·Langenbeck's Archives of Surgery·Ulrich C LienerMarkus W Knöferl
Nov 13, 2012·PLoS Neglected Tropical Diseases·Carolina RamírezDiane McMahon-Pratt
Nov 5, 1997·Journal of Pain and Symptom Management·M Anbar, B M Gratt
Mar 19, 2002·Cellular Signalling·Claudette Klein
Jan 25, 2003·Burns : Journal of the International Society for Burn Injuries·Martin G Schwacha
Jul 15, 1998·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·M Anbar, B M Gratt
Jul 22, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Roberto NicoleteLúcia Helena Faccioli
Sep 24, 2021·PLoS Pathogens·Courtney E McDougalJohn-Demian Sauer
Feb 8, 2006·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·N Ni Choileain, H P Redmond

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