PMID: 45221Jan 1, 1979

Effect of pH and buffers on insulin binding to normal and neoplastic mammary cells, fat cells and membrane preparations

Cancer Biochemistry Biophysics
S M ShafieR Hilf

Abstract

As a function of buffer pH, [125I]-insulin binding to rat mammary cells, rat adipocytes, or membranes prepared therefrom, at 4 degrees or 20 degrees C, showed 2 peaks in different buffers. Specific insulin binding at the pH 7.7. peak (100 +/- 11%) was lower than at pH 8.8 (140 +/- 17%) with no change in nonspecific binding. Although insulin stimulation of glucose uptake into fat cells was highest at pH 7.5, this response was also seen at pH 8.6. Scatchard affinity profiles, or in the kinetics of dissociation. Insulin degradation (< 10%) and binding to insulin antibody were similar over the pH range of 7 to 9.

Related Concepts

Buffers
Fat Pad
Tissue Membrane
Mammary Gland
Glucose, (beta-D)-Isomer
Glucose Import
Insulin Binding
Breast
Novolin
Insulin Receptor beta Subunit

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