PMID: 8675739Mar 1, 1996

Effect of pH changes in cerebrospinal fluid specimens on bacterial survival and antigen test results

Journal of Clinical Pathology
J G CunniffeM H Wilcox

Abstract

To determine the effect of pH changes occurring in cerebrospinal fluid (CSF) after sampling on the viability of meningitis causing bacteria, and on the performance of agglutination assays used for the rapid detection of bacterial antigens. The pH of CSF collected via lumbar puncture was measured by various methods, and the effects of the following different incubation conditions on subsequent changes were determined: air at 4 degrees C; air at room temperature (22 degrees C); air at 37 degrees C; and air with 5% CO2 at 37 degrees C. The growth/survival in pooled CSF of 15 bacterial isolates collected from 74 patients with meningitis was assessed in these incubation conditions over 24 hours. The effects of pH changes in the CSF on the sensitivity of two latex agglutination and one co-agglutination kits for detecting Haemophilus influenzae, Neisseria meningitidis groups B and C, and Streptococcus pneumoniae were determined. The measured pH of CSF was highly affected by the method used and particularly the time delay between patient sampling and assay. Measured pH values at the time of sampling (mean 7.5) increased rapidly within 60 seconds by about one unit. CSF pH continued to increase during incubation in all tested conditions ...Continue Reading

References

Mar 15, 2011·Journal of Neurology·Marieke T de GraafPeter A E Sillevis Smitt
Sep 4, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·J R StrawnT D Geracioti
Jul 29, 2008·The Journal of Antimicrobial Chemotherapy·Robert SauermannMarkus Zeitlinger
Jul 23, 2009·The Journal of Antimicrobial Chemotherapy·Robert SauermannMarkus Zeitlinger
Nov 13, 2014·Tidsskrift for den Norske lægeforening : tidsskrift for praktisk medicin, ny række·Inger Marie Waal NilsbakkenRaymond Jakobsen
Jan 3, 2012·Research in Microbiology·Richard SchwameisRobert Sauermann
May 14, 2011·Cytometry. Part B, Clinical Cytometry·Marieke T de GraafJan W Gratama
Nov 20, 1998·The Journal of Hospital Infection·M H Wilcox
Oct 15, 2011·Clinical Chemistry·Therese RosenlingRainer Bischoff
Nov 6, 2015·Molecular Neurodegeneration·Marco BarbarigaMassimo Alessio
Dec 14, 2019·Brain : a Journal of Neurology·Kaj BlennowKina Höglund
Mar 20, 2020·Veterinary Clinical Pathology·Lindsey N PetersonStephanie A Thomovsky
Nov 19, 2010·Gerodontology·Mario Augusto BrondaniHong Feng
Mar 8, 2011·Applied and Environmental Microbiology·Yury ShamisElena P Ivanova

Citations

Jan 1, 1976·Journal of Applied Physiology·D G Davies
Apr 1, 1992·Clinical Microbiology Reviews·L D Gray, D P Fedorko
Jan 1, 1991·Transactions of the Royal Society of Tropical Medicine and Hygiene·J E SippelZ Farid
May 1, 1986·Journal of Clinical Microbiology·R W SteeleC R Steele
Jun 1, 1995·Journal of Clinical Microbiology·M D PerkinsL B Reller

Related Concepts

Neisseria meningitidis
Haemophilus influenzae
Meningitis, Haemophilus
Spinal Puncture
Cerebrospinal Fluid pH
Coagglutination
Group B Streptococcal Pneumonia
Antigen Assay
Antigens, Bacterial
Specimen Collection

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