Effect of pH on endotoxin affinity for metal-ceramic alloys

The Journal of Prosthetic Dentistry
K L KnoernschildS D Campbell

Abstract

Crevicular pH may modify bacterial endotoxin affinity for high-noble metal-ceramic alloys. Porphyromonas gingivalis lipopolysaccharide (LPS) affinity for 3 metal-ceramic alloys at 3 different pH levels was compared in vitro by measuring adsorption to and release from the alloy surface. Metallographically polished disks were fabricated from Pd-Ag-Sn, Au-Pd-Ag-Sn-In, and Au-Pd-In-Ga alloys. Clean disks were placed individually into 1 mL at pH 6.5, 7.0, or 7.5 phosphate-buffered saline solution containing 0.9 endotoxin units per square millimeter tritiated LPS (n = 3 disks per alloy-pH group). The disks were incubated for 24 hours at 37 degrees C before being transferred to LPS-free buffer and incubated, again for 24 hours at 37 degrees C, to evaluate elution. This transfer continued at 24-hour intervals up to 96 hours total elution incubation. Lipopolysaccharide adsorption to and elution from disks was determined through liquid scintillation spectrometry. Adsorption data were evaluated with a 2-way analysis of variance (alpha=.05) and the post hoc Tukey honestly significant difference test. Lipopolysaccharide adsorption values ranged from 0.48 +/- 0.04 EU/mm(2) for the Au-Pd-Ag-Sn-In alloy at pH 7.5 to 0.75 +/- 0.04 EU/mm(2) for ...Continue Reading

References

Mar 1, 1977·Journal of Periodontal Research·S S SocranskyJ van Houte
Apr 1, 1977·Journal of Periodontology·J Valderhaug, L A Heloe
Aug 1, 1978·Journal of Clinical Periodontology·J E Highfield, R N Powell
Feb 1, 1979·The Journal of Prosthetic Dentistry·L M Jameson
Oct 1, 1979·Journal of Clinical Periodontology·J Slots
Jun 1, 1976·Journal of Periodontology·B P Sackett, R R Gildenhuys
Jan 1, 1976·Scandinavian Journal of Dental Research·J Slots
Apr 1, 1976·The Journal of Prosthetic Dentistry·C F Parkinson
Jun 1, 1975·Journal of Dental Research·E HausmannN Weinfeld
May 1, 1992·Journal of Periodontology·L A ChristerssonJ J Zambon
Feb 1, 1992·The Journal of Prosthetic Dentistry·M A FreilichR J Simonsen
Jan 1, 1991·Archives of Oral Biology·F M EggertM Goldner
Jan 1, 1991·The Journal of Prosthetic Dentistry·J D BaderD L Ramsey
Mar 1, 1991·The Journal of Prosthetic Dentistry·D A FeltonG P Wirthman
Mar 1, 1990·Journal of Clinical Periodontology·M QuirynenD van Steenberghe
Apr 1, 1986·Scandinavian Journal of Dental Research·S E SorensenK D Jörgensen
Apr 1, 1987·The Journal of Prosthetic Dentistry·D A OrkinD Bradshaw
Jan 1, 1966·Journal of Periodontal Research·E TheiladeH Löe
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L M WahlG R Martin
May 1, 1972·Journal of Periodontology·J SchwartzR B Parker
Oct 1, 1972·Journal of Periodontology·L ShapiroR E Stallard
Jan 1, 1974·Journal of Clinical Periodontology·W MörmannH H Renggli
Jan 1, 1972·Calcified Tissue Research·E HausmannW A Miller
Jan 1, 1971·Journal of Periodontology·N Gilmore, A Sheiham
Apr 1, 1971·Archives of Oral Biology·C G Hatfield, A Baumhammers
Dec 1, 1971·The Journal of Prosthetic Dentistry·M L Perel
May 1, 1984·Journal of Periodontal Research·A C TannerJ M Goodson
Feb 1, 1983·Journal of Periodontology·F A DeRenzis, S Y Chen
Feb 1, 1980·The Journal of Prosthetic Dentistry·M P KeenanC K Wade
Oct 1, 1980·Journal of Periodontology·M K Jeffcoat, T H Howell
Nov 1, 1980·The Journal of Prosthetic Dentistry·J Ehrlich, N Hochman

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Citations

Oct 29, 2013·The Journal of Prosthetic Dentistry·Valentim Adelino Ricardo BarãoCortino Sukotjo
Mar 15, 2016·The Journal of Prosthetic Dentistry·Weiqiang YuSongmei Zhang

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