PMID: 6167223Dec 1, 1980Paper

Effect of pH on lipolysis, cAMP and cAMP-dependent protein kinase activity in isolated rat fat cells

Archives internationales de physiologie et de biochimie
G E Chiappe de Cingolani, F V Vega

Abstract

The effect of acidosis and alkalosis on lipolysis, cAMP production and cAMP-dependent protein kinase activity in isolated rat fat cells incubated in the presence of norepinephrine and norepinephrine plus theophylline has been investigated. The pH of the incubation medium was adjusted to 6.8, 7.4 and 7.8 respectively. Acidosis inhibited both norepinephrine- and norepinephrine plus theophylline-induced release of glycerol whereas alkalosis led to slight stimulation. Norepinephrine produced an increase in cAMP and cAMP-dependent protein kinase activity. However, comparison of both parameters in acidosis and alkalosis with those at pH 7.4 indicates that they were higher at pH 7.8 and lower at pH 6.8. Addition of theophylline in combination with norepinephrine increases cAMP production within 5 min, under acidosis to values similar to those obtained at pH 7.4 with norepinephrine. The same effect on protein kinase activity was obtained. In spite of this increment in cAMP and protein kinase activity produced by addition of norepinephrine plus theophylline, lipolysis remains inhibited by acidosis. Addition of theophylline at pH 7.4 and 7.8 induced a much higher cAMP production and cAMP-dependent protein kinase activity although at pH 7...Continue Reading

References

Feb 1, 1976·Acta Physiologica Scandinavica·P Hjemdahl, B B Fredholm
Jul 1, 1974·The American Journal of Physiology·F V Vega, G E Chiappe de Cingolani
Aug 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·R O SCOWB B SMITH
Feb 1, 1951·The British Journal of Ophthalmology·D P GREAVES, E S PERKINS

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Citations

Jul 16, 2014·Journal of Dairy Science·Karen L WojciechowskiDavid M Barbano
Aug 21, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Daniela Rosendo-Silva, Paulo Matafome

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