PMID: 7016530Mar 1, 1981Paper

Effect of pH on pyrazole binding to liver alcohol dehydrogenase

European Journal of Biochemistry
P AnderssonG Pettersson

Abstract

1. Kinetic and equilibrium data have been determined at different pH between 4 and 10 for binding of the inhibitor pyrazole to liver alcohol dehydrogenase and to the binary complexes formed between enzyme and NADH or NAD+. 2. Pyrazole binding to free enzyme requires the protonated form of an ionizing group with a pKa of 9.2, agreeing with the pKa value reported for the water molecule bound at the catalytic zinc ion of the enzyme subunit. The rate of association of the inhibitor to the enzyme . NAD+ complex exhibits a similar pKa-7.6-dependence attributable to ionization of zinc-bound water in the latter binary complex. These observations lend support to the idea that pyrazole combines to the catalytic zinc ion on complex formation with the enzyme, zinc-bound water most likely being displaced by the inhibitor. 3. The rate of dissociation of the inhibitor from the ternary enzyme . NAD+ . pyrazole complex is proportional to the hydrogen ion concentration over the examined pH range (4-8). This effect of pH, which is proposed to reflect ionization of the enzyme-bound inhibitor with a pKa value below 4 (indirectly estimated to 2.4), accounts for the exceptional stability of the ternary complex at neutral and alkaline pH. It is conclu...Continue Reading

References

Jun 15, 1978·European Journal of Biochemistry·J Kvassman, G Pettersson
Oct 1, 1979·European Journal of Biochemistry·J Kvassman, G Pettersson
Oct 1, 1976·European Journal of Biochemistry·J Kvassman, G Pettersson
Dec 1, 1972·Canadian Journal of Biochemistry·P A GurrJ T Wong
Feb 1, 1980·European Journal of Biochemistry·J Kvassman, G Pettersson
Jan 1, 1981·European Journal of Biochemistry·P AnderssonG Pettersson

❮ Previous
Next ❯

Citations

Sep 1, 1982·European Journal of Biochemistry·D S SigmanR E Anderson
Apr 18, 2001·Chemico-biological Interactions·Y PockerC C Bhat
Jan 1, 1984·Archives of Biochemistry and Biophysics·C Syvertsen, J S McKinley-McKee
Mar 1, 1982·European Journal of Biochemistry·P Andersson, G Pettersson
Sep 1, 1983·Analytical Biochemistry·P J Lad, H L Leffert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.