Effect of pH on structure, function, and stability of mitochondrial carbonic anhydrase VA

Journal of Biomolecular Structure & Dynamics
Danish IdreesMd Imtaiyaz Hassan

Abstract

Mitochondrial carbonic anhydrase VA (CAVA) catalyzes the hydration of carbon dioxide to produce proton and bicarbonate which is primarily expressed in the mitochondrial matrix of liver, and involved in numerous physiological processes including lipogenesis, insulin secretion from pancreatic cells, ureagenesis, gluconeogenesis, and neuronal transmission. To understand the effect of pH on the structure, function, and stability of CAVA, we employed spectroscopic techniques such as circular dichroism, fluorescence, and absorbance measurements in wide range of pH (from pH 2.0 to pH 11.5). CAVA showed an aggregation at acidic pH range from pH 2.0 to pH 5.0. However, it remains stable and maintains its secondary structure in the pH range, pH 7.0-pH 11.5. Furthermore, this enzyme has an appreciable activity at more than pH 7.0 (7.0 < pH ≤ 11.5) with maximum activity at pH 9.0. The maximal values of kcat and kcat/Km at pH 9.0 are 3.7 × 10(6) s(-1) and 5.5 × 10(7) M(-1) s(-1), respectively. However, this enzyme loses its activity in the acidic pH range. We further performed 20-ns molecular dynamics simulation of CAVA to see the dynamics at different pH values. An excellent agreement was observed between in silico and in vitro studies. Th...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y GotoA L Fink
Jan 1, 1990·Methods in Enzymology·V S Stoll, J S Blanchard
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S J DodgsonL Mela
Sep 22, 1995·Journal of Molecular Biology·A S Yang, B Honig
May 26, 1995·Science·B Honig, A Nicholls
Feb 24, 1995·The Journal of Biological Chemistry·C PokalskyR L Van Etten
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y NagaoW S Sly
Mar 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J SaarnioW S Sly
May 23, 2001·Protein Science : a Publication of the Protein Society·K L ShawC N Pace
Feb 12, 2004·Journal of Molecular Recognition : JMR·Hans Rudolf BosshardIlian Jelesarov
Apr 6, 2004·Methods in Molecular Biology·Norma J Greenfield
Sep 24, 2005·The Journal of Chemical Physics·Jan Zielkiewicz
May 11, 2006·Chemical Reviews·Catherine A Royer
Apr 5, 2007·Nature Protocols·Norma J Greenfield
May 17, 2007·Current Topics in Medicinal Chemistry·Giuseppina De Simone, Claudiu T Supuran
Jun 26, 2007·Protein Science : a Publication of the Protein Society·John H MissimerWilfred F van Gunsteren
Mar 14, 2008·Current Pharmaceutical Design·Claudiu T Supuran
Mar 12, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Md Khurshid Alam KhanFaizan Ahmad
Jul 1, 2010·Proteins·Kemper Talley, Emil Alexov
Aug 10, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Md Khurshid Alam KhanFaizan Ahmad
Jul 9, 2013·Journal of Computational Chemistry·Jing Huang, Alexander D MacKerell
Nov 28, 2013·Journal of Biomolecular Structure & Dynamics·Khalid AnwerMd Imtaiyaz Hassan
Apr 23, 2014·Applied Biochemistry and Biotechnology·Parvez KhanMd Imtaiyaz Hassan
May 3, 2014·Biological & Pharmaceutical Bulletin·Nam Ah KimSeong Hoon Jeong
Sep 2, 2014·Journal of Biomolecular Structure & Dynamics·Md Anzarul HaqueFaizan Ahmad
Jan 15, 2015·Journal of Biomolecular Structure & Dynamics·Abdullah NaiyerFaizan Ahmad
Jul 26, 2015·Journal of Biomolecular Structure & Dynamics·Farha NazMd Imtaiyaz Hassan
Oct 1, 2015·Journal of Biomolecular Structure & Dynamics·Danish IdreesMd Imtaiyaz Hassan
Jan 5, 2016·Journal of Biomolecular Structure & Dynamics·Faez Iqbal KhanMd Imtaiyaz Hassan

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Citations

Apr 20, 2016·Journal of Biomolecular Structure & Dynamics·Amresh PrakashMd Imtaiyaz Hassan
May 14, 2016·Journal of Biomolecular Structure & Dynamics·Parvez KhanMd Imtaiyaz Hassan
Dec 23, 2017·Journal of Biomolecular Structure & Dynamics·Sunayana Begum SyedMd Imtaiyaz Hassan
Jul 15, 2020·Journal of Biomolecular Structure & Dynamics·Khalid A AlamryMd Imtaiyaz Hassan
Sep 10, 2019·Journal of Biomolecular Structure & Dynamics·Saman FatimaMd Imtaiyaz Hassan
Dec 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Taj MohammadImtaiyaz Hassan
Oct 31, 2020·European Journal of Medicinal Chemistry·Shubham KumarVikramdeep Monga
Dec 29, 2020·Journal of Biomolecular Structure & Dynamics·Pulala Raghuveer YadavPavan Kumar Pindi

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Methods Mentioned

BETA
circular dichroism

Software Mentioned

Discovery Studio
CHARMM
CAVA
GROMACS
Dichroweb K2D

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