PMID: 40033Aug 1, 1979

Effect of pH on the calcium metabolism of isolated rat kidney cells

The Journal of Membrane Biology
R K Studer, A B Borle


The effects of metabolic and respiratory acidosis and alkalosis on cellular calcium metabolism were studied in rat kidney cells dispersed with collagenase. In both types of acidosis, the intracellular pH, total cell calcium, and the cell relative radioactivity after 60 min of labeling are significantly depressed. Kinetic analysis of 45-ca desaturation curves shows that acidosis decreases all three cellular calcium pools and depresses calcium fluxes between the superficial and cytosolic pools and between the cytosolic and mitochondrial pools. In alkalosis the intracellular pH, the total cell calcium, and the cell relative radioactivity are significantly increased. Kinetic studies show that in alkalosis, only the mitochondrial pool is consistently increased. Calcium exchange between the mitochondrial and cytosolic pool is increased in metabolic alkalosis only. These results suggest that hydrogen ion is an important modulator of calcium metabolism, and that the intracellular pH rather than extracellular pH is the critical factor in determining the calcium status of cells during altered acid-base conditions.


Jan 1, 1982·The Journal of Membrane Biology·A B Borle
Jan 1, 1982·The Journal of Membrane Biology·A B BorleJ H Anderson
Feb 17, 1994·Biochimica Et Biophysica Acta·A GasbarriniA B Borle
Dec 1, 1988·Kidney International·P F ShanleyG C Johnson
Sep 1, 1990·Kidney International·V J CanzanelloN E Madias
Nov 1, 1995·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·S SabatiniM Spohn
Sep 1, 1980·Archives of Biochemistry and Biophysics·R K Studer, A B Borle
Aug 1, 1981·Circulation Research·R A AltschuldG P Brierley


Jan 1, 1976·The American Journal of Physiology·N Beck, S K Webster
Jun 1, 1975·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H BaurE Pfaff
Dec 1, 1975·Canadian Journal of Physiology and Pharmacology·R A Sutton, J H Dirks
Jul 1, 1976·The Journal of General Physiology·G Plishker, H J Gitelman
May 1, 1968·The American Journal of Physiology·A StruyvenbergA S Relman
May 1, 1971·The Journal of Physiology·P J Romero, R Whittam
Mar 1, 1966·The Journal of General Physiology·S WallachJ V Bellavia
Aug 1, 1962·The American Journal of Physiology·M B BURG, J ORLOFF
Dec 1, 1963·Journal of Cellular Physiology·A P CARVALHON PACE
Jun 1, 1931·The Journal of Clinical Investigation·R F FarquharsonJ C Aub
Aug 1, 1967·The Journal of Clinical Investigation·J LemannE J Lennon

Related Concepts

Carbonic Acid Ions
Acidosis, Respiratory
Cell Survival
Carbon Dioxide
Metabolic Acidosis
Hydrogen-Ion Concentration
Cytoplasmic Matrix

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.