PMID: 40033Aug 1, 1979

Effect of pH on the calcium metabolism of isolated rat kidney cells

The Journal of Membrane Biology
R K Studer, A B Borle

Abstract

The effects of metabolic and respiratory acidosis and alkalosis on cellular calcium metabolism were studied in rat kidney cells dispersed with collagenase. In both types of acidosis, the intracellular pH, total cell calcium, and the cell relative radioactivity after 60 min of labeling are significantly depressed. Kinetic analysis of 45-ca desaturation curves shows that acidosis decreases all three cellular calcium pools and depresses calcium fluxes between the superficial and cytosolic pools and between the cytosolic and mitochondrial pools. In alkalosis the intracellular pH, the total cell calcium, and the cell relative radioactivity are significantly increased. Kinetic studies show that in alkalosis, only the mitochondrial pool is consistently increased. Calcium exchange between the mitochondrial and cytosolic pool is increased in metabolic alkalosis only. These results suggest that hydrogen ion is an important modulator of calcium metabolism, and that the intracellular pH rather than extracellular pH is the critical factor in determining the calcium status of cells during altered acid-base conditions.

References

Jan 1, 1982·The Journal of Membrane Biology·A B Borle
Jan 1, 1982·The Journal of Membrane Biology·A B BorleJ H Anderson
Feb 17, 1994·Biochimica Et Biophysica Acta·A GasbarriniA B Borle
Dec 1, 1988·Kidney International·P F ShanleyG C Johnson
Sep 1, 1990·Kidney International·V J CanzanelloN E Madias
Nov 1, 1995·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·S SabatiniM Spohn
Sep 1, 1980·Archives of Biochemistry and Biophysics·R K Studer, A B Borle
Aug 1, 1981·Circulation Research·R A AltschuldG P Brierley

Citations

Jan 1, 1976·The American Journal of Physiology·N Beck, S K Webster
Jun 1, 1975·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H BaurE Pfaff
Dec 1, 1975·Canadian Journal of Physiology and Pharmacology·R A Sutton, J H Dirks
Jul 1, 1976·The Journal of General Physiology·G Plishker, H J Gitelman
May 1, 1968·The American Journal of Physiology·A StruyvenbergA S Relman
May 1, 1971·The Journal of Physiology·P J Romero, R Whittam
Mar 1, 1966·The Journal of General Physiology·S WallachJ V Bellavia
Aug 1, 1962·The American Journal of Physiology·M B BURG, J ORLOFF
Dec 1, 1963·Journal of Cellular Physiology·A P CARVALHON PACE
Jun 1, 1931·The Journal of Clinical Investigation·R F FarquharsonJ C Aub
Aug 1, 1967·The Journal of Clinical Investigation·J LemannE J Lennon

Related Concepts

Calcium
Carbonic Acid Ions
Kidney
Acidosis, Respiratory
Mitochondria
Cell Survival
Carbon Dioxide
Metabolic Acidosis
Hydrogen-Ion Concentration
Cytoplasmic Matrix

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