May 1, 1976

Effect of pH on the transient reduction of pig-plasma benzylamine oxidase by benzylamine derivatives

European Journal of Biochemistry
A LindströmG Pettersson

Abstract

1. The transient kinetics of reduction of the 470-nm absorption band in benzylamine oxidase by substrate at different pH values between 6 and 10 have been studied by stopped-flow techniques, and substituent effects on kinetic parameters for the reduction process have been examined using a series of ring-substituted benzylamine derivatives as the substrates. 2. Reduction of the enzyme by substrate takes place in two kinetically distinguishable steps, with the intermediate formation of an enzyme-substrate complex in which the substrate appears to be covalently bound through its amino group to the prosthetic group of the enzyme, possibly in the form of an amine-pyridoxal Schiff-base. 3. The apparent stability of the enzyme-substrate complex shows no obvious dependence on the electronic properties of the amine substrates, but is strongly pH-dependent in a way suggesting that substrate-binding involves the non-protonated amines, exclusively, and requires the presence of the acid form of an ionizing group in the enzyme with apparent pKa of 8.8. 4. Reduction of the enzymatic 470-nm chromophore and release of the aldehyde product of the catalytic process are rate-limited by the same monomolecular reaction step involving the enzyme-subs...Continue Reading

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Mentioned in this Paper

Benzylamine
Amines
Derivatives
Structure-Activity Relationship
Phenylmethylamine
Monoamine Oxidase
Benzylamine Oxidase
Aldehydes
Benzylamines
Hydrogen-Ion Concentration

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