Effect of pharmacological agents on human keratinocyte mitosis in vitro. II. Inhibition by catecholamines

Journal of Cellular Physiology
R A Harper, B A Flaxman


Catecholamines produce mitotic inhibition in primary cell cultures of human keratinocytes probably via a block in the G2 part of the cell cycle. Epinephrine produced significant mitotic inhibition (49%) at a concentration as low as 4.5 X 10(-10) M, while its analog, isoproterenol, produced 47% inhibition at 1 X 10(-10) M. Norepinephrine elicited a 49% inhibitory response at 1 X 10(-8) M. One other catecholamine, dopamine, caused a 53% decrease in mitosis at 1 X 10(-6) M. Other structurally related amines to exhibit mitotic inhibition were phenylephrine, 58% at 1 X 10(-7) M; octopamine, 47% at 1 X 10(-5) M; and tyramine, 52% at 1 X 10(-4) M. Serotonin showed no mitotic inhibition at 1 X 10(-4) M. Various alpha and beta adrenergic blocking agents were added to the cell system. The alpha blocking agent, phentolamine, had no effect on mitosis. When added in conjunction with epinephrine or norepinephrine, no reduction of the catecholamine-induced mitotic inhibition was observed. The beta blocking agent, propranolol, by itself showed slight mitotic inhibition at 1 X 10(-6) M. When added along with epinephrine or noreinephrine, propranolol reduced the catecholamine-induced mitotic inhibition approximately 65%. In addition, propranolol...Continue Reading


Oct 1, 1971·Archives of Dermatology·J A PowellJ J Voorhees
Apr 24, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·J WagnerD Reinhardt
Sep 15, 1971·Nature: New Biology·G O BronstadI Oye
Mar 1, 1972·Archives of Dermatology·J J VoorheesW H Kelsey
Jul 1, 1972·The Journal of Investigative Dermatology·J J VoorheesE R Harrell
Dec 1, 1973·The Journal of Cell Biology·C W Abell, T M Monahan
Feb 1, 1974·The British Journal of Dermatology·J J Voorhees, P D Mier
Jan 1, 1974·Annual Review of Biochemistry·P Cuatrecasas
Sep 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·R A HarperD P Chopra
Dec 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·R J Boucek, N L Noble
Apr 25, 1969·Science·P Molinoff, J Axelrod
Jul 1, 1972·The British Journal of Dermatology·D P Chopra, B A Flaxman
Jul 1, 1952·The Journal of Endocrinology·W S BULLOUGH


Apr 1, 1989·Agents and Actions·W A FogelC Maslinski
Jan 1, 1987·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·B Maurer-SchultzeE D Wachsmuth
Oct 2, 2007·Dermatologic Clinics·Raja K SivamaniR Rivkah Isseroff
Jul 22, 2004·Journal of Dermatological Science·Mehtap OzkurAhmet S Aynacioglu
Sep 28, 2005·The Journal of Investigative Dermatology·Shigeyoshi FuziwaraMitsuhiro Denda
Dec 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandra R VaughnR Rivkah Isseroff

Related Concepts

Dose-Response Relationship, Drug
Epinephrine Acetate
M Phase, Mitotic
Norepinephrine, (+, -)-Isomer

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.