PMID: 1201273Dec 16, 1975Paper

Effect of phase transition on the kinetics of dye transport in phospholipid bilater structures

Biochemistry
T Y Tsong

Abstract

Binding of 8-anilino-1-naphthalenesulfonate to dimyristoyl-L-alpha-lecithin bilayers enhances the fluorescence quantum yield of the dye molecule by 100-fold. By following the generation of fluorescence after a rapid mixing in a stopped-flow apparatus (mixing time 2 msec), kinetics of the binding of the fluorescence probe to the phospholipid vesicles has been investigated in the temperature range where the crystal-liquid crystal phase transition of the bilayer structures occurs. No reactions depending on the dye or the vesicle concentrations were detected. This suggests that the initial adsorption of the dye was very rapid. Two kinetic phases which appear in the 50 msec and the second time ranges are unimolecular. The faster one has a small amplitude and is observable in the entire temperature range studied. In the phase transition region the slower reaction becomes the major kinetic phase. It also increases the apparent concentration of bound dye by a factor of 2. These observations suggest that the 50-msec reaction has detected a reorientation of the probe molecule after the initial binding, and that the slow reaction represents a transport of the dye molecule into the inner layer of the lipid vesicle. The transport reaction i...Continue Reading

Citations

Feb 28, 1981·The Journal of Membrane Biology·M AhmedI W Kellaway
Jan 12, 1996·Biochimica Et Biophysica Acta·J L ThomasD A Tirrell
Feb 12, 1986·Biochimica Et Biophysica Acta·R C MaranhaoT G Redgrave
Jan 1, 1980·Journal of Biochemical and Biophysical Methods·J R Lakowicz
Jul 1, 1977·Photochemistry and Photobiology·W W Mantulin, H J Pownall
May 3, 2000·Journal of Biomechanical Engineering·S BhowmickJ C Bischof
Nov 1, 1989·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·C Watała, Z Jóźwiak
Mar 1, 1978·Journal of Cellular Physiology·B A Herman, S M Fernandez
Jun 9, 1981·Biochimica Et Biophysica Acta·M G Rockley, D S Najjar
Mar 17, 1977·Biochimica Et Biophysica Acta·D PapahadjopoulosR Lazo
Jan 27, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Lewen Yang, James T Kindt
Jul 12, 1977·Biochemistry·T Y TsongM I Kanehisa
Jun 14, 1977·Biochemistry·T Y Tsong, M I Kanehisa
Nov 25, 1980·Biochemistry·J B Swaney, B C Chang
Mar 17, 1981·Biochemistry·J Teissie, T Y Tsong
Sep 1, 1986·European Journal of Biochemistry·M Rögner, P Gräber
Jun 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Grażyna NeunertKrzysztof Polewski
Feb 24, 2021·The Journal of Membrane Biology·Antje Pokorny, Paulo F Almeida
Aug 11, 1982·Biochimica Et Biophysica Acta·J Slavík
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Grażyna NeunertKrzysztof Polewski
Nov 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Mark A KreutzbergerPaulo F Almeida
May 17, 2018·The Journal of Physical Chemistry. B·Rodrigo M Cordeiro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Biology of Lipids

Lipids are essential in many biological functions, including membrane structure, energy storage, and cell signaling. Find the latest research on the cell biology of lipids here.