Effect of phenobarbital on 7-Ethoxycoumarin-O-deethylase and microsomal epoxide hydrase activities in collagen gel cultures of rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
K De SmetV Rogiers

Abstract

Ethoxycoumarin-O-deethylase (ECOD) and microsomal epoxide hydrase (mEH) activities have been studied in collagen gel cultures of rat hepatocytes under control conditions and after exposure to phénobarbital. The hepatocytes were either sandwiched between two layers of extracellular matrix (type I collagen) or directly mixed with the same type of collagen gel. In preliminary experiments it was first tested whether it was possible to measure ECOD activity directly in intact hepatocytes that were embedded in a collagen gel matrix. Probably by a delay of substrate penetration through the collagen gel, the latter had to be removed by a collagenase digestion before ECOD activity measurements took place. When cultured in control medium, the activity of both phase I enzymes studied decreased as a function of culture time in sandwich as well as in immobilization cultures. After 4 days, however, a steady-state situation was reached and this level of enzymatic activity was observed for at least 2 wk. When the cells were exposed to 3.2 mm phenobarbital, an increased ECOD activity remained detectable for at least 14 days. In sandwich cultures a two- to threefold increase of ECOD activity was observed, whereas in immobilization cultures, incr...Continue Reading

References

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Citations

Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T NikiA Geerts
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T VanhaeckeV Rogiers
Nov 8, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T M Walker, A J Woodrooffe
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S BekenV Rogiers
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K De SmetV Rogiers
Jul 1, 1999·Alternatives to Laboratory Animals : ATLA·S CoeckeC Westmoreland

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