PMID: 9444341Jan 28, 1998Paper

Effect of phlebotomy technique on serum bicarbonate values

Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine
S D Berns, J L Matchett

Abstract

To determine whether the following phlebotomy technique factors result in spuriously low serum bicarbonate values: 1) small needle size, 2) prolonged tourniquet time, and 3) underfilling of Vacutainer tubes. Thirty-eight healthy young adult student volunteers from Brown University (mean age = 22 years) were randomly assigned to 1 of 2 (24-ga or 20-ga) IV needle size groups. With a tourniquet applied, the subjects had blood sampled initially and then at 3 intervals 1 minute apart. In addition, the initial blood sample was separated into four 4-mL Vacutainer tubes in blood volumes of 0.5, 1, 2, and 3 mL. Serum bicarbonate values (mmol/L) were compared using these techniques. 1) Small needle size did not affect serum bicarbonate values (p > 0.60); 2) prolonged tourniquet time resulted in a statistically significant serum bicarbonate elevation (32.0, 32.5, 33.8, and 33.6, respectively; p < 0.01); and 3) underfilling of Vacutainer tubes resulted in significantly lower serum bicarbonate values for all 4 Vacutainer blood volume groups (21.4, 23.0, 25.0, and 26.2, respectively; p < 0.001). Small needle size does not affect serum bicarbonate values. Prolonged tourniquet time results in a statistically significant elevation of serum bica...Continue Reading

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Citations

Nov 14, 2006·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Jill CorboPolly Bijur
Aug 24, 2013·Vascular Health and Risk Management·Chieko Fujii
Sep 28, 2012·Journal of the American Veterinary Medical Association·Stacy H TinklerPeter D Constable
Aug 19, 2011·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Philip GoldwasserRobert H Barth
Nov 25, 2016·Journal of the American Society of Nephrology : JASN·Matthew K Abramowitz
Jan 5, 2021·Emergency Medicine Australasia : EMA·Angela L ChiewJohn Mackenzie
Aug 18, 2020·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Tyler B WoodellDena E Rifkin

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