PMID: 1203324Jan 1, 1975Paper

Effect of phosphate and ionophores on (14C)-NEM incorporation in mitochondrial membranes and relationships with phosphate carrier system

Biochimie
Y BriandR Durand

Abstract

Phosphate transport in mitochondria was investigated with respect to its inhibition by NEM. The reactivity of the Pi carrier SH groups was influenced by phosphate or ionophores during preincubation before the addition of NEM. Furthermore in order to obtain some mitochondrial protein fractions where the typical effects of phosphate and ionophores on [14C]-NEM fixations were observed, mitochondria were submitted to hypotonic treatment and sonication. The following results were obtained: 1. -- Phosphate and grisorixin (a new ionophore of the nigericin group) decreased the inhibition of phosphate transport by NEM. The same effect was observed for [14C]-NEM incorporation. 2. -- Valinomycin increased [14C]-NEM incorporation. The valinomycin effect was abolished by phosphate. ClCCP alone affected [14C]-NEM incorporation slightly. Valinomycin plus ClCCP decreased NEM inhibition of phosphate transport and [14C]-NEM incorporation like grisorixin. 3. -- The variability of SH group reactivity can be interpreted by a control of SH group accessibility by transmembrane delta pH as previously suggested. 4. -- Typical effects of phosphate or ionophores were observed in whole pig heart and rat liver mitochondria. These effects were enhanced in t...Continue Reading

References

Jan 1, 1973·The Journal of Membrane Biology·H Rottenberg
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Aug 1, 1961·Analytical Biochemistry·E C WEINBACH

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Citations

Dec 1, 1978·Journal of Bioenergetics and Biomembranes·A Fonyó
Mar 1, 1984·European Journal of Biochemistry·E Ligeti, A Fonyó
Dec 1, 1981·Journal of Neurochemistry·A SalaminR W Straub
Jan 1, 1979·Pharmacology & Therapeutics·A Fonyó

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