Effect of Phosphate Limitation on Synthesis of Periplasmic Cyclic (beta)-(1,2)-Glucans.

Applied and Environmental Microbiology
M W BreedveldK J Miller

Abstract

Rhizobium meliloti and Agrobacterium tumefaciens synthesize periplasmic cyclic (beta)-(1,2)-glucans during adaptation to hypoosmotic environments. It also appears that these glucans provide important functions during the interactions of these bacteria with plant hosts. A large fraction of these glucans may become modified with anionic substituents such as phosphoglycerol or succinic acid; however, the role(s) of these substituents is unknown. In this study, we show that growth of these bacteria in phosphate-limited media leads to a dramatic reduction in the levels of phosphoglycerol substituents present on the periplasmic cyclic (beta)-(1,2)-glucans. Under these growth conditions, R. meliloti 1021 was found to synthesize anionic cyclic (beta)-(1,2)-glucans containing only succinic acid substituents. Similar results were obtained with R. meliloti 7154 (an exoH mutant which lacks the ability to succinylate its high-molecular-weight exopolysaccharide), revealing that succinylation of the cyclic (beta)-(1,2)-glucans is mediated by an enzyme system distinct from that involved in the succinylation of exopolysaccharide. In contrast, when A. tumefaciens C58 was grown in a phosphate-limited medium, it was found to synthesize only neutra...Continue Reading

References

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Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T DylanG Ditta

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Citations

Jan 10, 2002·Microbiology·Victoria J M AllanMarion Paterson-Beedle
Jun 12, 2013·Biopolymers·Ritika DwivediChristine M Szymanski
Nov 1, 1995·Journal of Bacteriology·M W BreedveldK J Miller
Mar 15, 2006·Applied and Environmental Microbiology·T S Al-NiemiT R McDermott

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