PMID: 11312896Apr 21, 2001Paper

Effect of phosphate on stability of pyridoxal in the presence of lysine

Journal of Agricultural and Food Chemistry
T C HuangC T Ho

Abstract

The stability of the biologically active compound vitamin B(6) in aqueous solution was investigated. Schiff base formation is the major reaction between the epsilon-amino group of lysine and the aldehyde group of both pyridoxal and pyridoxal phosphate. Model systems composed of equal molar concentrations of lysine with either pyridoxal or pyridoxal phosphate were used to study the effect of proton transfer on Schiff base formation. Pyridoxylidenelysine was found to be the major product in both lysine/pyridoxal and lysine/pyridoxal phosphate systems. Quantitation of residual pyridoxal and pyridoxal phosphate was conducted using an HPLC to evaluate the degradation of pyridoxal and pyridoxal phosphate. The results indicate both the free phosphate ion in the buffer system and the bound phosphate on pyridoxal phosphate can enhance the formation of the Schiff base. The phosphate group serves as both proton donor and acceptor, which catalyzes the Schiff base formation. The aldehyde group on pyridoxal phosphate was found to be much more reactive than that on pyridoxal. The bound phosphate group on pyridoxal phosphate, with proton donating and accepting groups in close proximity, can simultaneously donate and accept protons, thus enhanc...Continue Reading

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Citations

Jul 4, 2006·Chemico-biological Interactions·Flore DepeintPeter J O'Brien
Mar 20, 2018·Analytical and Bioanalytical Chemistry·Xiao-Fang WanGuang-Xue Chen
Mar 25, 2014·ACS Applied Materials & Interfaces·Sapana Kumari, Ghanshyam S Chauhan

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