Effect of phospholipase A2 on purified gastric vesicles

Journal of Supramolecular Structure
G SaccomaniG Sachs

Abstract

The phospholipid and fatty acid composition and role of phospholipids in enzyme and transport function of gastric (H+ + K+)-ATPase vesicles was studied using phospholipase A2 (bee venom). The composition (%) was phosphatidyl-choline (PC) 33%; sphingomyelin (sph) 25%; phosphatidylethanolamine (PE) 22%; phosphatidylserine (PS) 11%; and phosphatidylinositol (PI) 8%. The fatty acid composition showed a high degree of unsaturation. In both fresh and lyophilized preparations, even with prolonged incubation, only 50% of phospholipids were hydrolyzed, but the amount of PE and PS disappearing was increased following lyophilization. There was a marked decrease in K+-ATPase activity (75%) but essentially no loss of the associated K+ p-nitrophenyl phosphatase was found. ATPase activity could be largely restored by various phospholipids (PE greater than PC greater than PS). There was also an increase in Mg2+-ATPase activity, partially reversed in fresh preparations by the addition of phospholipids (PE greater than PS greater than PC). Proton transport activity of the preparation was rapidly inhibited, initially due to a large increase in the HCl permeability of the preparation. Associated with these enzymatic and functional changes, the ATP...Continue Reading

References

Jan 1, 1982·The Journal of Membrane Biology·G SachsE Rabon
Aug 7, 1986·Biochimica Et Biophysica Acta·A SoumarmonM J Lewin
Jul 4, 2001·European Journal of Biochemistry·F ScheirlinckxE Goormaghtigh
Nov 14, 1991·Biochemical and Biophysical Research Communications·A SmolkaR Swiger
Jun 1, 1982·Clinical Rheumatology·J P Famaey

Citations

Jan 21, 1977·Biochimica Et Biophysica Acta·H ChangG Sachs
Apr 22, 1977·The Journal of Membrane Biology·R SchackmannG Sachs
Aug 1, 1979·The Journal of Clinical Investigation·G SaccomaniG Sachs
Mar 23, 1979·Biochimica Et Biophysica Acta·J S Puskin, T Martin
Feb 25, 1977·Science·J E Rothman, J Lenard
Jan 17, 1972·Biochimica Et Biophysica Acta·G Meissner, S Fleischer
Dec 3, 1973·European Journal of Biochemistry·B Roelofsen, L L van Deenen
Apr 25, 1973·Biochimica Et Biophysica Acta·A L Ganser, J G Forte
Apr 25, 1973·Biochimica Et Biophysica Acta·C M ColleyL L van Deenen
Sep 14, 1971·Biochimica Et Biophysica Acta·B RoelofsenL L van Deenen
Sep 1, 1965·Life Sciences·V P SkipskiJ J Good

Related Concepts

Structure of Pyloric Gland
Protein Conformation
Electron Microscopy
H(+)-K(+)-Exchanging ATPase
Circular Dichroism, Vibrational
Stomach Diseases
DNA-dependent ATPase
Phospholipases A
Cell Surface Proteins
Phospholipase

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