PMID: 6403043Apr 13, 1983Paper

Effect of phospholipids on the regulation of a soluble galactosyltransferase in aortic wall

Biochimica Et Biophysica Acta
M Serres-Guillaumond, P Louisot

Abstract

A galactosyltransferase activity is located in the cell-sap of aortic intima-media cells. This enzymatic system calatyzes [14C]galactose transfer from UDP-[14C]galactose into endogenous and exogenous proteinic acceptors. Labelled products are isolated from the proteinic fraction obtained in 20% trichloroacetic acid pellet or from organic solvent extractions. Maximal [14C]galactose incorporation occurs at pH 7.8 in Tris-HCl buffer in the presence of 0.1 mM MnCl2 at 30 degrees C. The enzymatic activity is modified by phospholipids, particularly by phosphatidic acid and lysophosphatidylcholine, which behave as mixed inhibitors, while L-alpha-phosphatidylserine interacts as a competitive inhibitor. The effect of phospholipids is not stereospecific but appeared to be closely related to their polar headgroups, especially the acidic headgroups of phosphatidylcholine and phosphatidic acid. The chain length and the unsaturation degree of fatty acids involved in phospholipid structures are not a main factor of regulation. The lysophosphatidylcholine effect could be explained by its solubilization properties, as non-ionic detergents interact in the same way with galactosyltransferase activity. Exogenous phospholipids probably interact wit...Continue Reading

References

May 15, 1974·European Journal of Biochemistry·B S KhatraK Brew
Nov 1, 1974·Canadian Journal of Biochemistry·S Mookerjea, J W Yung
Jan 1, 1973·Advances in Protein Chemistry·R G Spiro
Oct 1, 1972·Journal of Molecular and Cellular Cardiology·M RichardP Louisot
Jan 24, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Guidollet, P Louisot
Aug 15, 1973·FEBS Letters·B B Kirschbaum, H B Bosmann
Jan 1, 1980·Canadian Journal of Biochemistry·S RatnamS Mookerjea
Oct 1, 1980·Canadian Journal of Biochemistry·M M Mitranic, M A Moscarello

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