Effect of physico-chemical modification on the immunogenicity of Haemophilus influenzae type b oligosaccharide-CRM(197) conjugate vaccines

Vaccine
Barbara BolgianoM J Corbel

Abstract

Haemophilus influenzae type b (Hib) poly-ribosyl-ribityl phosphate (PRP) oligosaccharide-CRM(197) conjugate vaccines from two different manufacturers (Hib A and Hib B) were subjected to adverse storage conditions and used to establish correlates between physico-chemical characteristics and immunogenicity. There were manufacturer-specific differences in the effect of freezing or freeze-thawing on the carrier protein conformation and the anti-CRM(197) or anti-PRP IgG response in rabbits whereas both conjugates showed similar stability when stored at elevated temperatures. Both oligosaccharide-CRM(197) conjugate vaccines formed apparent 'aggregates' of non-specifically associated higher molecular weight material when subjected to elevated temperatures or repeated freeze-thawing. Following subcutaneous injection of samples into CBA mice and New Zealand White rabbits, the amount of IgG raised against CRM(197) was significantly lower for samples incubated at 37 or 55 degrees C compared with those kept at 4 degrees C, consistent with the less well-folded conformation of the carrier protein observed at elevated temperatures. Moreover, there was a parallel reduction in the amount of IgG raised against PRP and the level of bactericidal a...Continue Reading

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Citations

Jul 22, 2015·Biologicals : Journal of the International Association of Biological Standardization·Robert B D OttoBarbara Bolgiano
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Fang GaoBarbara Bolgiano
Sep 21, 2017·Glycoconjugate Journal·Renata Chagas BastosIvna Alana Freitas Brasileiro da Silveira
Dec 7, 2019·Human Vaccines & Immunotherapeutics·Kelei LiZhenglun Liang

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