Effect of plasma from patients with idiopathic nephrotic syndrome on proteoglycan synthesis by human and rat glomerular cells

Pediatric Research
E P GirardinA De Agostini

Abstract

In vivo and in vitro findings have shown that plasma of patients with idiopathic nephrotic syndrome (INS) contain factors that increase glomerular permeability to proteins. The effects of these factors on proteoglycan synthesis by glomerular cells are unknown. To investigate the effect of plasma from patients with INS (n = 23) and other glomerulopathies (n = 12) on the amount of proteoglycans synthesized by cultured rat mesangial cells and human glomerular epithelial cells, glomerular cells were cultured for 24 h with plasma from patients or control subjects, and incorporation of Na2(35)SO4 in chondroitin dermatan sulfate and heparan sulfate was assessed using a cationic nylon membrane. The mean ratio of glycosaminoglycan produced by rat mesangial cells when in contact with plasma (5%) from INS patients to the amount produced when in contact with control plasma was 0.70+/-0.06. The mean ratio of heparan sulfate was 0.58+/-0.08. The decrease of heparan sulfate production was present in the cellular and in the extracellular fraction. It was observed when the cells were in contact with plasma from patients in relapse but not when in remission. No decrease of heparan sulfate production was observed with four of the five patients wi...Continue Reading

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Citations

Feb 17, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Nurcan CengizAli Anarat
May 11, 2005·Kidney International·Hicham MansourGeorges Deschênes
Dec 9, 2004·American Journal of Physiology. Renal Physiology·Anna BjörnsonJenny Sörensson
Jan 9, 2001·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·G Deschênes, L Martinat

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