Effect of (poly)-L-lactic acid on the proliferation and differentiation of primary bone cells in vitro

Journal of Biomedical Materials Research
T E OttoH J Haarman

Abstract

A previous study has shown bone formation around poly-L-lactic acid (PLLA) wire in vivo. However, it is still unknown how bone cells are stimulated to form bone around PLLA wire. The effect of PLLA on primary bone cells in vitro is the subject of this study. Osteoprogenitor and osteoblastic cells derived from neonatal mouse calvaria were cultured after addition of PLLA wire or L-lactide to the culture medium. Alkaline phosphatase (AP) activity, as a parameter of bone cell differentiation, and DNA content, to assess cell growth were measured. In osteoblast-enriched cell cultures PLLA wire did not affect DNA content, but AP activity was increased by 28%. In osteoprogenitor-enriched cell cultures PLLA wire decreased DNA content by 10%, but AP activity of the cells was not affected. L-Lactide enhanced the DNA content of osteoblastic cell cultures at 0.1 mM by 19%, but not at higher concentrations, and did not affect cell differentiation. In osteoprogenitor cell cultures, L-lactide had no effect at all. These results indicate that the proliferation and differentiation of bone cells in vitro can be modulated by PLLA, dependent on their stage of differentiation. L-Lactide at concentrations up to 10 mM did not adversely affect cell gro...Continue Reading

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Citations

Sep 5, 2002·Journal of Biomedical Materials Research·Kaiyong CaiHuiqi Xie
Nov 6, 2002·Journal of Biomedical Materials Research·Yukiharu HasegawaHideki Warashina
Apr 2, 2002·Journal of Biomaterials Science. Polymer Edition·K CaiX Li
May 11, 2002·Journal of Biomaterials Science. Polymer Edition·Kaiyong CaiHuiqi Xie
Jun 15, 2007·Development·Daniel BouvardReinhard Fässler
May 23, 2002·Journal of Biomaterials Science. Polymer Edition·K Isama, T Tsuchiya
Jan 17, 2009·Journal of Biomedical Materials Research. Part a·Yong ZhaoRacquel Z LeGeros

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