Effect of Polyethylene Glycol-Induced Molecular Crowding on the Enzymatic Activity and Thermal Stability of β-Galactosidase from Kluyveromyces lactis.

Journal of Agricultural and Food Chemistry
Verónica NolanMaría A Perillo

Abstract

Here, we report the effect of polyethylene glycol (PEG6000)-induced molecular crowding (MC) on the catalytic activity and thermal stability of Kluyveromyces lactis β-galactosidase (β-Gal). The β-Gal-catalyzed hydrolysis of o-nitrophenyl-β-d-galactopyranoside followed a Michaelian kinetics at [PEG6000] ≤ 25% w/v and positive cooperativity at higher concentrations (35% w/v PEG6000). Compared with dilute solutions, in the MC media, β-Gal exhibited stronger thermal stability, as shown by the increase in the residual activity recovered after preincubation at high temperatures (e.g., 45 °C) and by the slower inactivation kinetics. Considering the effects of water thermodynamic activity on the reaction kinetics and protein structure and the effect of the exclusion volume on protein conformation, we suggest that changes in the protein oligomerization state and hydration could be the responsible for the behavior observed at the highest MC levels assayed. These results could be relevant and should be taken into account in industrial food processes applying β-Gal from K. lactis.

References

Apr 1, 1980·Archives of Disease in Childhood·A HoskováR Pospísil
Mar 1, 1994·The International Journal of Biochemistry·R E HuberS K Khare
Dec 2, 1998·Journal of Biomolecular Structure & Dynamics·H A Scheraga
Mar 27, 2001·Protein Science : a Publication of the Protein Society·D K Eggers, J S Valentine
Oct 9, 2001·Trends in Biochemical Sciences·R J Ellis
Mar 20, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·J Matthew Hutzler, Timothy S Tracy
Nov 1, 2002·Biophysical Chemistry·Julieta M Sanchez, Maria A Perillo
Feb 21, 2003·Nature·R John Ellis
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Beatriz FarruggiaGuillermo Picó
Mar 23, 2004·Biochemical and Biophysical Research Communications·Chang Sup KimDeok-Kun Oh
Jun 9, 2005·Chemistry and Physics of Lipids·D Bach, I R Miller
Jul 11, 2006·Journal of Cell Science·Allen P Minton
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Jun 25, 2008·Annual Review of Biophysics·Huan-Xiang ZhouAllen P Minton
Sep 5, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Eduardo M ClopMaría A Perillo
Jan 1, 2010·Colloids and Surfaces. B, Biointerfaces·Marina C CrescimbeniMaría A Perillo
Mar 2, 2010·International Journal of Pharmaceutics·Wei WangDirk Teagarden
Jun 29, 2010·Journal of Bacteriology·Anja NenningerConrad W Mullineaux
Dec 24, 2011·Journal of Structural Biology·Angel Pereira-RodríguezJulia Sanz-Aparicio
Sep 11, 2012·The Journal of Physical Chemistry. B·Eduardo M ClopAna K Chattah
Mar 26, 2013·Colloids and Surfaces. B, Biointerfaces·Julieta M SánchezMaría A Perillo
Nov 21, 2013·Biochimica Et Biophysica Acta·Leonid BreydoVladimir N Uversky
Jan 27, 2015·Journal of Biomolecular Structure & Dynamics·Luisa A FerreiraBoris Y Zaslavsky
Jan 26, 2016·Journal of Agricultural and Food Chemistry·Jihua LiuJohn A Carver
Nov 2, 2018·Colloids and Surfaces. B, Biointerfaces·Sandra S FloresJulieta M Sánchez
Dec 1, 2018·Biochemical and Biophysical Research Communications·M Ines BurgosMaría A Perillo
May 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiang WuJie Zheng

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Citations

Mar 6, 2021·Biochemistry and Biophysics Reports·Xander E WilcoxKristin M Slade

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