Effect of polyethylene glycols on the alkaline-induced molten globule intermediate of bovine serum albumin

International Journal of Biological Macromolecules
Peng QuMaotian Xu

Abstract

In the present study, the formation of one molten globule-like unfolding intermediate of bovine serum albumin (BSA) at pH 11.2 has been established with the help of circular dichroism (CD) spectra, fluorescence spectroscopy and 'phase diagram' approach. Additionally, we have shown the conformational changes occurring in the pH 11.2 intermediate of BSA when it was exposed to different molecular weight polyethylene glycols (PEGs) at varying concentrations. When the pH 11.2 intermediate of BSA was treated by PEG 400 there was induction of secondary and non-native tertiary contacts. In case of PEG 8000 and PEG 20,000, the loss in secondary as well as tertiary structure was observed. PEG 8000 and 20,000 altered the conformation of the pH 11.2 intermediate and resulted in its transition to another intermediate state in which the hydrophobic patches were inaccessible.

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Citations

Jun 4, 2014·Journal of Photochemistry and Photobiology. B, Biology·Sudeshna GhoshSwagata Dasgupta
May 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiang WuJie Zheng
Oct 16, 2020·Protein Science : a Publication of the Protein Society·Seyed Hamidreza ArabiDariush Hinderberger
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zahoor Ahmad ParrayAsimul Islam

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