Effect of Polyhydroxybutyrate (PHB) storage on L-arginine production in recombinant Corynebacterium crenatum using coenzyme regulation

Microbial Cell Factories
Meijuan XuZhenghong Xu

Abstract

Corynebacterium crenatum SYPA 5 is the industrial strain for L-arginine production. Poly-β-hydroxybutyrate (PHB) is a kind of biopolymer stored as bacterial reserve materials for carbon and energy. The introduction of the PHB synthesis pathway into several strains can regulate the global metabolic pathway. In addition, both the pathways of PHB and L-arginine biosynthesis in the cells are NADPH-dependent. NAD kinase could upregulate the NADPH concentration in the bacteria. Thus, it is interesting to investigate how both PHB and NAD kinase affect the L-arginine biosynthesis in C. crenatum SYPA 5. C. crenatum P1 containing PHB synthesis pathway was constructed and cultivated in batch fermentation for 96 h. The enzyme activities of the key enzymes were enhanced comparing to the control strain C. crenatum SYPA 5. More PHB was found in C. crenatum P1, up to 12.7 % of the dry cell weight. Higher growth level and enhanced glucose consumptions were also observed in C. crenatum P1. With respect to the yield of L-arginine, it was 38.54 ± 0.81 g/L, increasing by 20.6 %, comparing to the control under the influence of PHB accumulation. For more NADPH supply, C. crenatum P2 was constructed with overexpression of NAD kinase based on C. crenat...Continue Reading

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Citations

Jan 16, 2019·Applied Microbiology and Biotechnology·Akhilesh Kumar SinghSatarudra Prakash Singh
Jul 18, 2019·Journal of Industrial Microbiology & Biotechnology·Jianli WangXiaoyuan Wang
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·Xiao-Yu WuBang-Ce Ye
Sep 12, 2018·World Journal of Microbiology & Biotechnology·Lei Han, Bo Liang
Dec 15, 2020·The Science of the Total Environment·Ayesha Algade AmaduSufeng Wang
Jun 21, 2021·Bioresource Technology·Bhoomika YadavPatrick Drogui

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Methods Mentioned

BETA
PCR
Protein Assay

Software Mentioned

ABI Prism 7000

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