Effect of Polyhydroxybutyrate/Chitosan/Bioglass nanofiber scaffold on proliferation and differentiation of stem cells from human exfoliated deciduous teeth into odontoblast-like cells

Materials Science & Engineering. C, Materials for Biological Applications
Maryam KhoroushiAbbas Ali Khademi

Abstract

Scaffolds and their characteristics play a central role in tissue engineering. The purpose of this study was to determine the effects of Polyhydroxybutyrate (PHB)/Chitosan/nano-bioglass (nBG) nanofiber scaffold made using the electrospinning method, on the proliferation and differentiation of stem cells obtained from human exfoliated deciduous teeth into odontoblast-like cells. In this experimental study, the pulps of the molten deciduous teeth were isolated, thereafter, the stem cells from human exfoliated deciduous teeth (SHED) were extracted and then the 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability percentage. The expression of some stem cell genes was studied by flowcytometry. These cells were then subjected to odontoblast by using the bone morphogenetic proteins-2 (BMP2) growth factor in the differentiation medium and for the expression of their specific genes. Primers of collagen type-I, dentin sialophosphoprotein (DSPP) and alkaline phosphatase (ALP) were used and the percentage of differentiation to odontoblast cells in induction scaffolds was investigated using real-time PCR and immunohistochemistry methods. The results revealed a 6-fold increase in the ...Continue Reading

Citations

Aug 24, 2018·Journal of Functional Biomaterials·Ludovica ParisiGuido M Macaluso
Nov 6, 2020·International Journal of Biological Macromolecules·Sanaz Soleymani Eil BakhtiariElahe Bahremandi Toloue
Jul 10, 2021·Frontiers in Cell and Developmental Biology·Bo LiYi Men
Aug 28, 2021·International Journal of Molecular Sciences·Zhuo XieZhengmei Lin

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