Effect of polyunsaturated fatty acids on diphenyl hydantoin-induced genetic damage in vitro and in vivo

Prostaglandins, Leukotrienes, and Essential Fatty Acids
Shivani PonnalaUndurti N Das

Abstract

Phenytoin sodium/diphenyl hydantoin (DPH) is used by a major segment of epileptics and neuro surgery patients with head injury to prevent seizures. DPH is a known mutagen, carcinogen, and teratogen. Essential fatty acids (EFAs) are critical for various tissues and were reported to act as anti-mutagenic agents. In the present study we assessed the effect of five EFAs on DPH-induced genetic damage both in vitro and in vivo. DPH induced significant genetic damage. Of all the EFAs (linoleic acid, alpha-linolenic acid, gamma-linolenic acid, arachidonic acid, dihomo-gamma-linolenic acid, and eicosapentaenoic acid) studied, all except eicosapentaenoic acid showed significant decrease in DPH induced genetic damage as assessed by micronucleus (MN) test. However, gamma-linolenic acid (GLA) was found to be the most effective in reducing the number of MN containing lymphocytes both in vitro and in vivo to control values. EFAs when tested alone produced insignificant increase in the amount of genetic damage but when tested in combination with DPH the number of micronuclei containing lymphocytes was reduced; but the DNA ladder pattern, an indication of DNA damage, was increased. This apparently paradoxical action of EFAs, especially of GLA, ...Continue Reading

References

Jan 1, 1987·Toxicology and Applied Pharmacology·N C MargarettenR A Coulombe
May 1, 1996·Journal of Pediatric Hematology/oncology·J C MurrayR L Hurwitz
May 30, 2002·Biological Chemistry·Dean G TangJames P Kehrer
Mar 15, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Päivi Peltomäki
Sep 15, 2004·Mutation Research·K Iravathy GoudY R Ahuja
Aug 8, 2006·Biotechnology Journal·Undurti N Das
Dec 16, 2006·Current Pharmaceutical Biotechnology·Undurti N Das

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Citations

May 4, 2000·Nutrition·U N Das
Jun 28, 2012·Progress in Neuro-psychopharmacology & Biological Psychiatry·Undurti N Das
Oct 22, 2011·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Undurti N Das
Feb 10, 2012·Toxicology and Industrial Health·Hasan Turkez, Elanur Aydin

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