Jul 29, 2009

Effect of polyurethane chemistry and protein coating on monocyte differentiation towards a wound healing phenotype macrophage

Joanne E McBaneRosalind S Labow


Tissue regeneration alternatives for peripheral vascular disease are actively being investigated; however, few studies in this area have probed the role of the wound healing monocyte-derived macrophage (MDM). Inflammatory MDMs transition to wound healing MDMs as the relative levels of tumor necrosis factor-alpha (TNF-alpha) decrease and IL-10 increase. TNF-alpha has been linked to the regulation of HMGB1 (high mobility group box 1 protein), a nuclear protein that upon macrophage stimulation can be secreted and act as a pro-inflammatory cytokine. This study investigated the influence of a degradable polar hydrophobic ionic polyurethane (D-PHI) on MDM cell expression of pro- versus anti-inflammatory markers, when the material was uncoated or pre-coated with collagen prior to cell studies. Effects were compared to similar groups on tissue culture polystyrene (TCPS). Collagen coated TCPS and D-PHI had significantly more DNA than the uncoated TCPS after 7d (p=0.001 and p=0.006 respectively); however, there was significantly less esterase activity from cells on D-PHI (+/-collagen) than for cells on TCPS after 7d (p=0.002, p=0.0003 respectively). No significant differences in esterase activity were observed between collagen coated and...Continue Reading

Mentioned in this Paper

Pathologic Cytolysis
Neuro-Oncological Ventral Antigen 2
Biochemical Pathway
Methylene dimethanesulfonate
HMGB1 gene
Tumor Necrosis Factor-alpha
Nuclear Proteins
Mitogen-Activated Protein Kinases
Coated Materials, Biocompatible
Inflammation Mediators

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