Effect of pore diffusion limitation on dextrin hydrolysis by immobilized glucoamylase

Biotechnology and Bioengineering
D D LeeY Y Lee

Abstract

Data reported here and previously indicate that when dextrin is hydrolyzed in the presence of immobilized glucoamylase, use of a larger average molecular weight substrate leads to lower overall rates of hydrolysis, while the maltose concentration during the bulk of the reaction and the maximum glucose concentration are lower than when the soluble form of the enzyme is employed under the same conditions. Computer simulation of the system demonstrated that all three observations were caused by pore diffusion limitation: the first by slow diffusion of substrate, the second by slow diffusion of intermediates, and the third by slow diffusion of glucose. Follow-up experiments with glucoamylase immobilized to particles of different sizes confirmed this finding, as results with the smallest beads were identical to those with soluble glucoamylase.

References

Feb 1, 1976·Biotechnology and Bioengineering·D D LeeG T Tsao
Nov 1, 1977·Biotechnology and Bioengineering·S J SwansonC H Lim

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Citations

Apr 15, 1992·Biotechnology and Bioengineering·K A Sims, M Cheryan
Aug 20, 1989·Biotechnology and Bioengineering·M M MeagherP J Reilly
Nov 25, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Olaf KruseBen Hankamer
May 20, 1988·Biotechnology and Bioengineering·P J SliningerT P Abbott
Jan 1, 1990·Journal of Chemical Technology and Biotechnology·M I SisoD Combes
Feb 1, 1984·Biotechnology and Bioengineering·S AdachiK Hashimoto
Feb 1, 1982·Biotechnology and Bioengineering·K A Ram, K Venkatasubramanian
Apr 1, 1982·Biotechnology and Bioengineering·V S Patwardhan, N G Karanth

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