Effect of porous structure of macroporous polymer supports on resolution in high-performance membrane chromatography of proteins

Journal of Chromatography. a
M B TennikovF Svec

Abstract

The effect of porous structures of 2-mm thick diethylamine functionalized monolithic polymethacrylate discs on their chromatographic behavior in ion-exchange mode has been studied. Discs with small pores did not perform well because they exhibited high back pressure and substantial peak broadening. Discs characterized with pores larger than 1,000 nm did not provide good separations either because the time required for some protein molecules to traverse the length across the pore to reach the wall for adsorption/desorption process that is essential for the separation may be longer than their residence time within the matrix. Optimum pore size is centered at about 700 nm. Excellent separations have been achieved with these discs even at very steep gradients and high flow-rates which allow to shorten the separation times substantially.

References

Apr 24, 1992·Journal of Chromatography·M NachmanP Bailon
Apr 24, 1992·Journal of Chromatography·M Nachman
Jan 24, 1992·Journal of Chromatography·D JosićW Reutter
Feb 7, 1992·Journal of Chromatography·P Langlotz, K H Kroner
Jul 27, 1984·Journal of Chromatography·X Geng, F E Regnier
Jul 20, 1988·Biotechnology and Bioengineering·A JungbauerE Wenisch

❮ Previous
Next ❯

Citations

Sep 27, 2012·Analytical and Bioanalytical Chemistry·E A PonomarevaT B Tennikova
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·H ZouD Zhou
Apr 14, 1999·Journal of Biotechnology·J HagedornT Tennikova
Jun 19, 2002·Journal of Biotechnology·H P Hentze, M Antonietti
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Natalia D OstryaninaTatiana B Tennikova
Oct 29, 2011·Journal of Chromatography. a·Yun Yang, Xindu Geng
Feb 26, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xindu Geng, Lili Wang
Nov 23, 2006·Journal of Chromatography. a·I V KalashnikovaT B Tennikova
Sep 10, 2004·Journal of Separation Science·Fatima M PlievaBo Mattiasson
Sep 10, 2004·Journal of Separation Science·Alfred ZöchlingAlois Jungbauer
Feb 6, 2009·Biotechnology Progress·Todd J Menkhaus, Jane Roseland
Oct 26, 2012·Biotechnology and Bioengineering·Oliver HardickDaniel G Bracewell
Nov 1, 2012·Journal of Separation Science·Fei WangXindu Geng
Jul 26, 2006·Advances in Colloid and Interface Science·Marcus Liew Kai HoaYong Zhang
Mar 19, 2015·Journal of Biotechnology·Oliver HardickDaniel G Bracewell
May 17, 2012·Journal of Separation Science·Alexander KorolevAlexander Kurganov
May 14, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jixia WangXinmiao Liang
Mar 24, 2005·Journal of Chromatography. a·Galina A Platonova, Tatiana B Tennikova
May 15, 2018·Sensors and Actuators. B, Chemical·Xianming KongAlan X Wang
Apr 4, 2021·Membranes·Solomon Mengistu LemmaRuben G Carbonell
Sep 3, 1998·Analytical Chemistry·R GiovanniniT B Tennikova
Aug 1, 1999·Analytical Chemistry·A PodgornikT Tennikova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.