PMID: 8600119Sep 1, 1995Paper

Effect of postmortem autolysis on Na,K-ATPase activity and antigenicity in the gerbil cochlea

Hearing Research
D A VincentB A Schulte

Abstract

Alterations in the enzymatic activity and antigenicity of Na,K-ATPase as well as changes in cochlear morphology were assessed in gerbil inner ears harvested at selected time intervals up to 18 h postmortem. Na,K-ATPase activity was assayed biochemically in one cochlea from each animal and the other cochlea was fixed and embedded in paraffin for evaluation by light microscopy. Na,K-ATPase antigenicity was assessed by immunostaining with a broad-spectrum antiserum reactive with all known isoforms of the enzyme, and structural preservation was evaluated on adjacent sections stained with hematoxylin and eosin. The results showed a downward trend in enzymatic activity of Na,K-ATPase in lateral wall tissues within 1 h of death. In contrast, Na,K-ATPase immunoreactivity was fairly well preserved with postmortem fixation delays up to 12 h, despite the considerable structural degradation of cochlear tissues which began 2-3 h postmortem. It is concluded that under controlled environmental conditions, cochleas collected up to 4 h postmortem are suitable for morphological and immunohistochemical study of Na,K-ATPase by light microscopy. Cochleas collected more than 5 h postmortem were useful only for relatively gross immunohistochemical st...Continue Reading

References

Jul 1, 1990·Hearing Research·J H MillsL F Kulish
Feb 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B A Schulte, J C Adams
Sep 1, 1958·The Laryngoscope·C FERNANDEZ

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Citations

Feb 24, 2001·The Laryngoscope·C D CunninghamB N Schmiedt
Jan 28, 2003·Journal of Speech, Language, and Hearing Research : JSLHR·Flint A Boettcher
Jan 25, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Simone N WeisCarlos A Netto

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